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- Medium: | - Medium: | ||
*100ml of distilled water | *100ml of distilled water | ||
*2g of agar | *2g of agar | ||
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*Meat extract 3 g | *Meat extract 3 g | ||
*Made up with distilled water to 1000 ml | *Made up with distilled water to 1000 ml | ||
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[[File:wd11.JPG|400px]] | [[File:wd11.JPG|400px]] | ||
* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | * Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | ||
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CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | ||
'''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | '''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | ||
* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''. | * Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''. | ||
[[File:wd12.JPG|400px]] | [[File:wd12.JPG|400px]] | ||
* Day 2: I made a new sample with a new medium | After 5 days the samples of ''Polycephalum/physarum'' din't came to live again. | ||
[[File:wd23.jpg|400px]] | |||
* Day 2: I made a new sample with a new medium. Parallel to the samples that I use for the experiment I started to use this new Medium to grow the specimen (to have live healthy samples to experiment), I started from the formula that Fernanda Caicedo gets to in her previous experiments, and I altered for my special use. | |||
Medium: | Medium: | ||
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*(mix together) | *(mix together) | ||
*2 gr of agar | *2 gr of agar | ||
*(mix all together and | *(mix all together and boil) | ||
At left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample. | |||
[[File:pp-1.jpeg|400px]] | [[File:pp-1.jpeg|400px]] | ||
* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non salty medium and the one in the salty medium seems that have died. | * Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non-salty medium and the one in the salty medium seems that have died. | ||
[[File:pp-2.jpg|400px]] | [[File:pp-2.jpg|400px]] | ||
*Day 7: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have the red color. | |||
[[File:wd24.jpg|400px]] | |||
NOTE: Samples with the pink/red contamination/mutation/reaction. Other samples that I'm growing using the same made medium and the same original Petri dish with the specimen don't have this situation. I don't have an explanation for this fenomena in the moment. | |||
[[File:wd25.jpg|400px]] | |||
[[File:wd26.jpg|400px]] | |||
*Day 8: A made some new medium ''(this time for the salinity medium I just used 2% of salinity concentration)'' and made a new experiment, this time in the divided medium I used just agar at the left and the salinity 2% concentration medium at left. Two control samples at left with the ideal medium for the growth of the specimen and at the right full concentration of salinity in all the Petri dish. | |||
[[File:wd27.jpg|400px]] | |||
After one night the specimen in the ideal medium is already growing, the specimen in the middle medium is not moving like the last time ''(probably because this time the food is concentrated in the salinity medium)'' and the samples in the salinity medium seem to be dying again. | |||
[[File:wd28.jpg|400px]] |
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