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- Medium: | - Medium: | ||
100ml of distilled water | |||
2g of agar | |||
Leaflets | |||
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- Medium: | - Medium: | ||
NaCl 30 g | |||
Glycerol 1 g | |||
Pepton (Bacxto-peptone) 10 g | |||
Meat extract 3 g | |||
Made up with distilled water to 1000 ml | |||
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* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | * Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | ||
CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | ||
'''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | '''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | ||
* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''. | * Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''. | ||
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* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample. | * Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample. | ||
Medium: | Medium: | ||
*100 ml of water | *100 ml of water | ||