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- Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, lives as a symbiont in the light organ of certain fish and squid species. Grows at temperatures of 24°. | - Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, lives as a symbiont in the light organ of certain fish and squid species. Grows at temperatures of 24°. | ||
- The bioluminescence of A. | - The bioluminescence of A. Fischeri is caused by transcription of the lux operon, which is induced through population-dependent quorum sensing. The population of this specimen needs to reach an optimal level to activate the lux operon and stimulate light production. | ||
- Many people have work with this specimen trying to make their own version of the medium. Common ingredients in most media include Tryptone, yeast extract, glycerol, and roughly 3% NaCl by weight. | - Many people have work with this specimen trying to make their own version of the medium. Common ingredients in most media include Tryptone, yeast extract, glycerol, and roughly 3% NaCl by weight. | ||
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[[File:wd12.JPG|400px]] | [[File:wd12.JPG|400px]] | ||
After 5 days the samples of ''Polycephalum/physarum'' didn't | After 5 days the samples of ''Polycephalum/physarum'' didn't come to live again, my first hypothesis is that the medium was too salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state). | ||
[[File:wd23.jpg|400px]] | [[File:wd23.jpg|400px]] | ||
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[[File:pp-2.jpg|400px]] | [[File:pp-2.jpg|400px]] | ||
*Day 7: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have | *Day 7: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have a red color. | ||
[[File:wd24.jpg|400px]] | [[File:wd24.jpg|400px]] | ||
NOTE: Samples with the pink/red contamination/mutation/reaction. Other samples that I'm growing using the same made medium and the same original Petri dish with the specimen | NOTE: Samples with the pink/red contamination/mutation/reaction. Other samples that I'm growing using the same made medium and the same original Petri dish with the specimen doesn't have this situation. I don't have an explanation for this phenomena at the moment. | ||
[[File:wd25.jpg|400px]] | [[File:wd25.jpg|400px]] | ||
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[[File:wd35.jpg|400px]] | [[File:wd35.jpg|400px]] | ||
*Day 12: | *Day 12: Definitely the samples in the salinity medium are death. Ant the contamination/chemical reaction continues in the other samples. | ||
[[File:wd36.jpg|400px]] | [[File:wd36.jpg|400px]] | ||
* Day 15: Because the salinity in the medium is | * Day 15: Because the salinity in the medium is too high for the specimens to growth, I reduced the salinity inside the medium into .3% in order to see the reaction of the specimen. The right samples continue to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium. | ||
[[File:wd37.jpg|400px]] | [[File:wd37.jpg|400px]] | ||
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*Day 19: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination. | *Day 19: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination. | ||
Because of health conditions and the use that can be given in the laboratory where I work, I put away | Because of health conditions and the use that can be given in the laboratory where I work, I put away these samples (disposed of them in a clean way). | ||
<gallery> | <gallery> | ||
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*Day 53: | *Day 53: | ||
I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and | I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and in order to start the test of adaptation again. | ||
[[File:wd53.jpg|400px]] | [[File:wd53.jpg|400px]] | ||
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*Day 55: | *Day 55: | ||
With these new healthy samples of the specimen, I made two | With these new healthy samples of the specimen, I made two Petri dishes in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium. | ||
[[File:wd51.jpg|400px]] | [[File:wd51.jpg|400px]] | ||
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*Day 59: | *Day 59: | ||
I create new petri | I create new petri dishes the half is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 0.12% of salinity (there I situated the food). | ||
[[File:wd59.jpg|400px]] | [[File:wd59.jpg|400px]] | ||
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*Day 63: | *Day 63: | ||
I create new petri | I create new petri dishes the half is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 0.24% of salinity (there I situated the food). | ||
[[File:wd63.jpg|400px]] | [[File:wd63.jpg|400px]] | ||
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*Day 65: | *Day 65: | ||
I create new petri | I create new petri dishes the half is the previous environment with 0.24% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 0.3% of salinity (there I situated the food). One of the previous samples got contamination so I continue with just one sample. | ||
[[File:wd66.jpg|400px]] | [[File:wd66.jpg|400px]] | ||
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*Day 69: | *Day 69: | ||
I create new petri | I create new petri dishes the half is the previous environment with 0.3% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 0.6% of salinity (there I situated the food). | ||
[[File:wd67.jpg|400px]] | [[File:wd67.jpg|400px]] | ||
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*Day 75: | *Day 75: | ||
I create new | I create new Petri dishes the half is the previous environment with 0.6% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 1% salinity (there I situated the food). | ||
[[File:wd69.jpg|400px]] | [[File:wd69.jpg|400px]] | ||
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*Day80: | *Day80: | ||
I create new | I create new Petri dishes the half is the previous environment with 1% of salinity (there I situated the Physarum polycephalum) | ||
and the other | and the other half is with 2% salinity (there I situated the food). | ||
[[File:wd71.jpg|400px]] | [[File:wd71.jpg|400px]] | ||
*Day | *Day 86: | ||
After | After six days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium. | ||
[[File:wd72.jpg|400px]] | |||
*Day90: | |||
I create new Petri dishes the half is the previous environment with 2% of salinity (there I situated the Physarum polycephalum) | |||
and the other half is with 3% salinity (there I situated the food). I also translated the specimens into new Petri dishes with their new level of salinity in order to keep the specimens healthy. | |||
'''CONCLUSIONS:''' | '''CONCLUSIONS:''' |
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