Paola S. Calderón/Project: Specimen ''Physarum Polycephalum''.: Difference between revisions

*Day 8: A made some new medium ''(this time for the salinity medium I just used 2% of salinity concentration)'' and made a new experiment, this time in the divided medium I used just agar at the left and the salinity 2% concentration medium at left. Two control samples at left with the ideal medium for the growth of the specimen and at the right full concentration of salinity in all the Petri dish.

* Day 15: Because the salinity in the medium is too high for the specimens to growth, I reduced the salinity inside the medium into .3% in order to see the reaction of the specimen. The right samples continue to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium.

* Day 17: For the ideal medium the specimen is growing, but after a week it acquire some mode, the middle sample move to the food area away from the salinity medium, and the specimen in the salinity medium started to move in order to try to find food. It is tolerating the salinity medium. The specimen in the salinity medium with 0.3% concentration of NaCl is starting to get into hibernation mode, I put some food in order to make it strong before transplanting it into a new medium with higher concentrations of NaCl.

*Day 20: I continue increasing the salinity quantities in the medium of the specimen to 0.4% and 0.5%. It is working.  

I try without success to revive my trained samples of Physarum polycephalum. My hypothesis is that the medium where I tried to revive them was not the one with the salinity concentration where they were taught before that they will find food. Sadly I don't have more samples of the taught samples in .04Nacl to prove my theory.  

With these new healthy samples of the specimen, I made two Petri dishes in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium.

I create new petri dishes the half is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum)  

I create new petri dishes the half is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum)  

I create new petri dishes the half is the previous environment with 0.3% of salinity (there I situated the Physarum polycephalum)  

After six days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium.  

and the other half is with 3% salinity (there I situated the food). I also translated the specimens into new Petri dishes with their new level of salinity in order to keep the specimens healthy.