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| *[[/ The third Idea of physarum project]] | | *[[/ The third Idea of physarum project]] |
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| ==My Final Project==
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| ==='''Concept'''===
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| This section is devoted to those non-scientists (or fledgling scientists) who are interested in simple methods for culturing Physarum.
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| Some of the documentation included here is not easily referenced, having come from websites that are no longer available, or having been obtained via web-surfing (e.g. Google) that yielded material in one search but not another. Wherever possible I have included, in the document, the URL from which it was obtained and/or an acknowledgment of the source. I apologize for any inadvertent infringement on copyright or "intellectual property" but ask forgiveness since the sole purpose of this section is to encourage the non-expert - especially young students - to give it a try and HAVE SOME FUN.
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| ==='''Process'''===
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| In this part I would like to show the process of a project from the beginning to the end.
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| *Life Cycle of Physarum polycephalum [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/Life_Cycle_of_Physarum_polycephalum]
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| *A Simple Method of Growing the Plasmodial Slime Mold Physarum Polycephalum [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/A_Simple_Method_of_Growing_the_Plasmodial_Slime_Mold_Physarum_Polycephalum]
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| *Growing Plasmodia [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/Growing_Plasmodia]
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| ====''Materials''====
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| *Petri dish (glass or plastic, need not be sterile) or other suitable container.
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| *Paper towels (white is best, brown will work).
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| *Old Fashioned Oatmeal Flakes (not the instant).
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| *Tap water.
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| *A plasmodium or sclerotium-available from biological supply houses.
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| *Medium
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| **100 ml of distilled water
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| **2g of agar
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| **oat flakes
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| ====''Methods''====
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| Cover container bottom with piece of paper towel.
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| *Place piece of living plasmodium or sclerotium on paper towel.
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| *Moisten paper towel with tap water and drain off excess water.
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| *Add one oat flake near slime mold.
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| *Place lid over dish and place in a dark place at room temperature (e.g. place in a drawer or closet).
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| *Next day examine culture and sprinkle with water to give Physarum a gentle rinse and pour off excess water (rinsing improves growth). *Add 1-3 additional oat flakes near advancing front of plasmodium.
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| *Continue to examine, rinse, and feed daily or every few days.
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| ''Additional cultures are easily made by transferring a plasmodial covered oat flake to another container. Producing several cultures is desirable to ensure that you don't loose an only culture due to a unforeseen failure of a plasmodium. Also, having more than one culture allows you to test the effects of different treatments on the slime mold (e.g. exposure to light, cold, different food sources, etc.).''
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| ==='''Final Project'''===
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| Now you can see the photos of final project.
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| <gallery>
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| File:13.JPG|Preparation of Medium
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| File:14.JPG|Cook Medium in Microwave
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| File:17.JPG|Preparation of Project
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| File:20.JPG|After 1 Day
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| File:19.JPG|After 1 Day
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| File:36.JPG|Documentation of mein Project
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| File:2.JPG|After 2 Days
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| File:51.JPG|After 3 Days
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| File:4.JPG|After 6 Days
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| File:49.JPG| After 8 Days
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| File:3.JPG|After 10 Days
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| File:5.JPG||After 15 Days
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| File:6.JPG|After 20 Days
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| File:7.JPG|After 25 Days
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| File:61.JPG|After 25 Days
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| File:8.JPG|After 30 Days
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| File:66-1.jpg|After 45 Days
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| File:68.JPG|The outgoing physarum from the bottle
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| File:69.JPG|The outgoing physarum from the bottle
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| File:70.JPG|The outgoing physarum from the bottle
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| </gallery>
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| ==='''Refrences'''===
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| here are the references of a project this semester. [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/References ]
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