421
edits
GMU:Introduction to Microscopy/Denise Nicoau (view source)
Revision as of 13:23, 15 November 2020
, 15 November 2020→Introduction to Microscopy | WiSe - Denise Nicolau
No edit summary |
|||
| Line 33: | Line 33: | ||
* immersion oil: able to increase resolution (applicable if you have a 100x objective lens) | * immersion oil: able to increase resolution (applicable if you have a 100x objective lens) | ||
* fixation: it refers to the process of attaching cells to a slide; it is often achieved either by heating or chemically treating the specimen. It kills microorganisms in the specimen, stopping their movement and metabolism while preserving the integrity of their cellular components for observation | * fixation: it refers to the process of attaching cells to a slide; it is often achieved either by heating or chemically treating the specimen. It kills microorganisms in the specimen, stopping their movement and metabolism while preserving the integrity of their cellular components for observation | ||
* simple staining: a single dye is used to emphasize particular structures in the specimen | |||
* differential staining: more dyes are used to emphasize particular structures in the specimen | |||
| Line 88: | Line 90: | ||
* eosin | * eosin | ||
* rose bengal | * rose bengal | ||
Simple staining vs. differential staining | |||
A simple stain will generally make all of the organisms in a sample appear to be the same colour, even if the sample contains more than one type of organism. In contrast, differential staining distinguishes organisms based on their interactions with multiple stains. In other words, two organisms in a differentially stained sample may appear to be different colours. | |||
''Differential staining techniques commonly used'' | |||
* Gram staining | |||
* acid-fast staining | |||
* endospore staining | |||
* flagella staining | |||
* capsule staining. | |||