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GMU:Flagelates, Nematodes, and I/Nino Schmidt (view source)
Revision as of 13:30, 14 January 2021
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== Project == | == Project == | ||
I am trying to find out how nematode Heterorhabditis bacteriophora are reacting to salt and sugar. I'm trying to find out if they prefer one environment more or even die in one. The nematodes stay in petri dishes with water, as we have found out that this is where they have the best chance of survival | I am trying to find out how nematode Heterorhabditis bacteriophora are reacting to salt and sugar. I'm trying to find out if they prefer one environment more or even die in one. The nematodes stay in petri dishes with water, as we have found out that this is where they have the best chance of survival. | ||
These following articles inspired me: | |||
[https://homeguides.sfgate.com/use-sugar-garden-78219.html Sugar in the Garden] | |||
and | |||
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796247/ The Role of Sugar Transporter Genes during Early Infection by Nematodes] | |||
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'''first attempts to cook a medium in the laboratory:''' | '''first attempts to cook a medium in the laboratory:''' | ||
[[File:7703F625-2304-4A8E-82AC-E9C46FEF92ED_1_105_c.jpeg| | -> before i came up with the idea of the following experiment | ||
[[File:7703F625-2304-4A8E-82AC-E9C46FEF92ED_1_105_c.jpeg|220px]] [[File:kökö.jpeg|220px]] [[File:8AC33DEC-A281-4E55-9B06-C0119481C108_1_105_c.jpeg|220px]] | |||
'''Project implementation idea:''' | '''Project implementation idea:''' | ||
I try to find nematodes in soil by dissolving the earth in water | I try to find nematodes in soil by dissolving the earth in water. | ||
''-> since i can't get any from the BioLab'' | |||
I am going to try to divide the namdeaths found in the earth into 4 petri dishes. | I am going to try to divide the namdeaths found in the earth into 4 petri dishes. | ||
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'''fourth petri dish:''' Nematodes and sugar on the on and salt on the other side of the petri dish with a little bit of water (to see if they prefer and move to one side) | '''fourth petri dish:''' Nematodes and sugar on the on and salt on the other side of the petri dish with a little bit of water (to see if they prefer and move to one side) | ||
-> Later I adapted the distribution of the petri dishes to the new circumstances that arose during the experiment (read in the protocol below) | |||
'''My first attempts to find Nematodes in soil:''' | '''My first attempts to find Nematodes in soil:''' | ||
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[[File:0C6AC3AB-9780-4BAD-BE47-548B2861CB71_1_105_c.jpeg|130px]] | [[File:0C6AC3AB-9780-4BAD-BE47-548B2861CB71_1_105_c.jpeg|130px]] This what you can see looking through the microscope with a smartphone camera | ||
-> unfortuantely i can see no Nematodes | -> unfortuantely i can see no Nematodes | ||
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''Because of that Miga is going to send me Nematodes to my post adress at home.'' | ''Because of that Miga is going to send me Nematodes to my post adress at home.'' | ||
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'''Day 1 with the Nematodes''' | |||
Today i received the Nematodes. I am going to disolve them in water (room temperature) and try to bring them back to life. As soon as they start to move i am going to contiune the expermient. | |||
'''Remark:''' If you are trying find nematodes in soil and isolate them afterwards as i tried in the first place you may find this website helpful: [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Nematodeisolation.pdf Nematode Isolation] | |||
[[File:packaged.jpeg|230px]] | |||
[[File:disolving in water.jpeg|230px]] | |||
[[File:disolved in water.jpeg|230px]] | |||
In the following video which i uploaded to Youtube you can see the Nematodes through a cellphone microscope. At that time, hoewever, they haven't moved yet.: | |||
[https://youtu.be/qBg0eJOhR_U Nematodes that don't move] | |||
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'''Day 2 with the Nematodes''' | |||
Today I tried to get the nematodes moving in differently tempered water. I tried it with hot, cold and water that I put in the refrigerator together with the nematodes. They didn't move / live in anyone. After that, I came up with the idea of looking at whether they are moving directly from the packaging when they are dry, with success! In the following YouTube video, I recorded them moving.: [https://youtu.be/nWdV-qX8Dws moving Nematodes] | |||
Now that I know that they don't seem to move or survive in water, I'm going to continue my experiment differently. I am going to disolve sugar in one and salt in another petri dish with water. than I'll wait for the water to evaporate. (the petri dishes should now have a layer of salt and sugar) After that i am going to put the nematodes without water on top of the dried disolved sugar and salt petri dishes. I'll see how the nematodes behave on the individual petri dishes and how long they stay alive. As a check, I'll also put nematodes in a clean petri dish. | |||
In the following picutres you can see how i disolved the sugar and salt. In order for the water to evaporate faster, i then put it an a heater. | |||
'''Remark:''' To prevent the petri dishes from beeing swapped, they are labaled! | |||
[[File:labeled petri dishes.jpeg|240px]] [[File:petri dishes with salt and water.jpeg|240px]] [[File:petri dishes on a heater.jpeg|240px]] | |||
I will contiune with the experiment as soon as the water has evaporated | |||