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GMU:Flagelates, Nematodes, and I/Nino Schmidt (view source)
Revision as of 13:30, 14 January 2021
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== Project == | == Project == | ||
I am trying to find out how nematode Heterorhabditis bacteriophora are reacting to salt and sugar. I'm trying to find out if they prefer one environment more or even die in one. The nematodes stay in petri dishes with water, as we have found out that this is where they have the best chance of survival. | I am trying to find out how nematode Heterorhabditis bacteriophora are reacting to salt and sugar. I'm trying to find out if they prefer one environment more or even die in one. The nematodes stay in petri dishes with water, as we have found out that this is where they have the best chance of survival. | ||
These following articles inspired me: | |||
[https://homeguides.sfgate.com/use-sugar-garden-78219.html Sugar in the Garden] | |||
and | |||
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796247/ The Role of Sugar Transporter Genes during Early Infection by Nematodes] | |||
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Today I tried to get the nematodes moving in differently tempered water. I tried it with hot, cold and water that I put in the refrigerator together with the nematodes. They didn't move / live in anyone. After that, I came up with the idea of looking at whether they are moving directly from the packaging when they are dry, with success! In the following YouTube video, I recorded them moving.: [https://youtu.be/nWdV-qX8Dws moving Nematodes] | Today I tried to get the nematodes moving in differently tempered water. I tried it with hot, cold and water that I put in the refrigerator together with the nematodes. They didn't move / live in anyone. After that, I came up with the idea of looking at whether they are moving directly from the packaging when they are dry, with success! In the following YouTube video, I recorded them moving.: [https://youtu.be/nWdV-qX8Dws moving Nematodes] | ||
Now that I know that they don't seem to survive in water | Now that I know that they don't seem to move or survive in water, I'm going to continue my experiment differently. I am going to disolve sugar in one and salt in another petri dish with water. than I'll wait for the water to evaporate. (the petri dishes should now have a layer of salt and sugar) After that i am going to put the nematodes without water on top of the dried disolved sugar and salt petri dishes. I'll see how the nematodes behave on the individual petri dishes and how long they stay alive. As a check, I'll also put nematodes in a clean petri dish. | ||
In the following picutres you can see how i disolved the sugar and salt. In order for the water to evaporate faster, i then put it an a heater. | In the following picutres you can see how i disolved the sugar and salt. In order for the water to evaporate faster, i then put it an a heater. | ||