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GMU:Different Worlds/Jesse Lucjan Stegmann (view source)
Revision as of 00:51, 12 June 2021
, 12 June 2021→Technical steps
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29.5. | |||
I make room on the top shelf of my laboratory and manically disinfect the surfaces, as well as everything I touch the surfaces with. At least the laboratory is at an unusually comfortable height for precision work. | I make room on the top shelf of my laboratory and manically disinfect the surfaces, as well as everything I touch the surfaces with. At least the laboratory is at an unusually comfortable height for precision work. | ||
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While trying to prepare the media and the petri-dishes in a decidedly un-sterile environment, I suddenly understand how people become germ-fearing, compulsively washing neat freaks. From cooking the agar in an old kitchen pot, to repeatedly disinfecting everything that somehow physically comes into contact with anything else over and over, always feeling I have broken the chain of sterility somewhere along the line. | |||
'''False Starts | |||
''' | |||
Paula, who donated her Physarum Sample, informs me she gave me the wrong petri-dish by accident. When I open the foil around the dish, I see what she means. The dish consists almost entirely of mold, only at the very edge there are traces of Physarum. | |||
My friend from Berlin, who also provided some special media to experiment with, added two dishes of BM5, which as he tells me, is especially nutritious and used for plant embryos. However, he also had a mishap, one of the two dishes already had a big mold in it, when it arrived. I realise I will most likely have to get another batch of Physarum, but decide to make the most of it. I attempt to isolate the traces from the edge of the dish as good as I can and implant them into newly prepared agar dishes. | |||
Since the damage is done, I cut away the mold from the BM5, and implant some Physarum on it too, just to see if how it behaves. Luckily I still have another, uncontaminated BM5 medium that I save for when I eventually obtain an uncontaminated Physarum. | |||
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