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== Making the substrate == | == Making the substrate == | ||
For the substrate we followed this guide. | For the substrate we followed this guide. | ||
[[File:Euglena_sheet.jpg|300px]] | |||
We decided to make 500ml of the solution so we could have a few tries from the beginning in case some of them failed. Working with live organisms is a lot of trial and error. | We decided to make 500ml of the solution so we could have a few tries from the beginning in case some of them failed. Working with live organisms is a lot of trial and error. | ||
For that I first did some calculations and multiplied all the necessary ingredient times five, since the recipe was originally for 100ml of solution. I wrote everything down to make it easier for myself once I actually got to weigh everything with the scales. | For that I first did some calculations and multiplied all the necessary ingredient times five, since the recipe was originally for 100ml of solution. I wrote everything down to make it easier for myself once I actually got to weigh everything with the scales. | ||
[[File:notebookentry.jpg|300px]] | |||
I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | ||
I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. |
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