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I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | ||
I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | ||
I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. | I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker. | ||
After preparing the solutions in their respective bottles we put them in the pressure cooker to kill off any spores or bacteria that might have landed in them while working (Make sure the lid of the bottle is on the bottle but not screwed all the way shut to prevent the glass from breaking in the pressure cooker. Also make sure somebody is there to watch the pressure cooker for workplace safety. Alessandro kindly did that for us while we went and got lunch). | After preparing the solutions in their respective bottles we put them in the pressure cooker to kill off any spores or bacteria that might have landed in them while working (Make sure the lid of the bottle is on the bottle but not screwed all the way shut to prevent the glass from breaking in the pressure cooker. Also make sure somebody is there to watch the pressure cooker for workplace safety. Alessandro kindly did that for us while we went and got lunch). | ||
Once everything was nice and sterile we prepared our workbench for making the actual substrate and put some Euglena in there for growing. We prepared all the solutions and put them where I can easily reach them. I also got the worksheet ready so I know how much has to go in the base solution | Once everything was nice and sterile we prepared our workbench for making the actual substrate and put some Euglena in there for growing. We prepared all the solutions and put them where I can easily reach them. I also got the worksheet ready so I know how much has to go in the base solution, the vitamin solution and the Euglena sample to make sure I can work quickly and efficiently without having to search for things when I'm already working on it. Then I desinfected my hands, put gloves on and desinfect them again. Since we made more of the base solution I also had to take that into account when dropping in the other solutions. After the full substrate was done I filled four plastic containers until about 3/4 full and added a few of the drops with Euglena in them. Then we put all the tubes in a stand near a window to make sure the Euglena could photosynthesize and grow. Since it's winter Alessandro put up a UV lamp for them. | ||
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File:preparing euglena 1.jpg | |||
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File:preparing euglena12.jpg | |||
</gallery> | |||
== Ideas for using Euglena == | == Ideas for using Euglena == |
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