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==Identification (Antje)== | ==Identification (Antje)== | ||
Another goal of the workshop was to try to roughly identifiy what was growing on the petri dishes. Zachery was providing us with a [http://www.endophyte.club/how-to/5-identify table] that gave a very basic idea of what could be | Another goal of the workshop was to try to roughly identifiy what was growing on the petri dishes. Zachery was providing us with a [http://www.endophyte.club/how-to/5-identify table] that gave a very basic idea of what could possibly be found or what to look for. More or less a differentiation between Form, Elevation and Margin could be made. We tried to identifiy ours. The most obvious differentiation one could achieve with the eyes or by observing with the microscope would be to identify wether the cultures are bacterial origin or fungal. | ||
Fungal colonies and bacterial colonies in culture on a petri dish can differ in terms of their appearance, texture, and growth patterns. Under a microscope, fungal colonies typically appear as fluffy, white or off-white mats with a texture that is soft and sometimes cottony. Bacterial colonies, on the other hand, often appear as smooth, round or irregularly shaped, and opaque. Bacterial colonies may also have a more dense appearance compared to fungal colonies. | |||
In terms of growth patterns, fungal colonies tend to spread outwards in a radial fashion and can form complex branching structures. Bacterial colonies, on the other hand, tend to grow in a circular fashion and do normally not form the same types of branching structures as fungi. | |||
These differences can be used to distinguish between fungal and bacterial colonies under the microscope. However, it's important to note that some species of bacteria can form complex, three-dimensional structures, while some fungal species may form colonies that are relatively smooth and circular, making a definitive identification based solely on appearance challenging in some cases. | |||
===Conclusion=== | |||
So what was again clear after the workshop there are several methods that can be used to definitively identify a specimen as either fungal or bacterial in nature. Some of these include: | |||
Microscopy: Examination of the microscopic structure of the colony can provide information about the nature of the organism. This may include observation of cell shape, size, and arrangement, as well as the presence of structures such as hyphae (in fungi) or flagella (in some bacteria). | |||
Staining techniques: Different staining techniques can be used to highlight specific features of the cells and can aid in the identification of the organism. For example, Gram staining is commonly used to differentiate between Gram-positive and Gram-negative bacteria. | |||
Molecular methods: DNA-based techniques, such as polymerase chain reaction (PCR) and sequencing, can be used to identify the organism to the species level. This may involve amplifying and sequencing specific genetic markers, such as the ribosomal DNA, that are characteristic of a particular group of organisms. | |||
Culture characteristics: The way in which the colony grows on a petri dish can also provide information about the nature of the organism. This may include the rate of growth, the formation of aerial structures, and the ability to produce pigments or other metabolic products. | |||
It is important to note that a combination of these methods may be required for a definitive identification, as some organisms may be difficult to classify based on a single criterion. In addition, some organisms may be misclassified due to contamination or other factors, so it is important to confirm the identity of the specimen through multiple lines of evidence. | |||
But anyhow. As and Artist it is a good start to be curious and be inspired by the different shapes and colors. And to use this as a creative source for other projects. |
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