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→Day 1: (6h)
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*Learn to cultivate a mushroom in vitro | *Learn to cultivate a mushroom in vitro | ||
*Document the experiment | *Document the experiment | ||
[[Documentation- Day 1 / 17.04.2023]] | |||
After Alessandro explained the rules in the laboratory and the different functions of the equipment to us, we were now allowed to start working ourselves. We prepared our work materials, for which we needed a jar with a perforated lid, a bit of cotton wool, a scale, a petri dish, a measuring cup, as well as malt extract, distilled water, and agar agar powder. | |||
(Photos of the Equipment ) | |||
We were also given a table that allowed us to determine the perfect ratio of ingredients for a perfect nutrient medium | |||
(photos table) | |||
We mixed 0.2 g of malt and 0.2 g of agar agar with 10 ml of liquid. Everything was mixed together and put into the jar. | |||
The malt extract mixed with agar agar and distilled water provides a perfect, soft nutrient medium for the organisms. | |||
(Photos jar) | |||
Next, we needed to sterilize everything inside and outside of the jar, so we placed the lids (equipped with cotton wool in the perforation to prevent any mold from getting inside) into the pressure cooker. One of the jars was marked with tape that would change its color, so we would know when the pressure cooker had finished its sterilization process. | |||
(photos tape, jars in cooker) | |||
After the jars were finished, we noticed the first changes. Due to the heat, the agar agar had turned from a liquid to a solid form. Now, we were able to inoculate our nutrient medium with the mushrooms. | |||
( photos) | |||
For this, we used a suction device. After disinfecting all surfaces and preparing everything, we were ready to start. The following materials were needed: disinfectant, gloves, a Bunsen burner, a scalpel, the mushroom cultures, and our jars. | |||
The jar with the nutrient medium was already slightly opened, the scalpel was disinfected over the Bunsen burner, cooled down a bit in the nutrient medium of the other jars and then a rectangle was cut out from the edge/border of the mycellium and placed into the prepared jar. After these steps were completed, the jars were placed in the incubator. | |||
===Day 2: (6h)=== | ===Day 2: (6h)=== | ||