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GMU:BioArt Forum/Ornella Del Monaco (view source)
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For this workshop we focused on learning a molecular biology technique called PCR used to repeatedly replicate (amplify) a defined stretch of DNA for which the starting and ending nucleotide sequences are known. It is necessary to prepare samples (plants, flower, fruit, saliva, skin) of substances whose DNA we want to replicate and we do this through a process of mushing and mixing and centrifuging. After the peparation of the samples, we prepare the gel that need to contain them. Once pipeting the samples in the gel we wait for them to give results. Already after half an hour we can understand which samples contain DNA and in which the stretch of DNA is long or short. | For this workshop we focused on learning a molecular biology technique called PCR used to repeatedly replicate (amplify) a defined stretch of DNA for which the starting and ending nucleotide sequences are known. It is necessary to prepare samples (plants, flower, fruit, saliva, skin) of substances whose DNA we want to replicate and we do this through a process of mushing and mixing and centrifuging. After the peparation of the samples, we prepare the gel that need to contain them. Once pipeting the samples in the gel we wait for them to give results. Already after half an hour we can understand which samples contain DNA and in which the stretch of DNA is long or short. | ||
[[File:W2 p1 .jpg|left|thumb|314x314px]] | [[File:W2 p1 .jpg|left|thumb|314x314px]] | ||
[[File:W2 p2.jpg | [[File:W2 p2.jpg|thumb|335x335px|[[File:W2 p3.jpg|left|thumb|[[File:W2 vid 1.mp4|left|thumb]]]]]] | ||