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== 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet == | == 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet == | ||
For this workshop we focused on learning a molecular biology technique called PCR used to repeatedly replicate (amplify) a defined stretch of DNA for which the starting and ending nucleotide sequences are known. It is necessary to prepare samples (plants, flower, fruit, saliva, skin) of substances whose DNA we want to replicate and we do this through a process of mushing and mixing and centrifuging. After the peparation of the samples, we prepare the gel that need to contain them. Once pipeting the samples in the gel we wait for them to give results. Already after half an hour we can understand which samples contain DNA and in which the stretch of DNA is long or short. | For this workshop we focused on learning a molecular biology technique called PCR used to repeatedly replicate (amplify) a defined stretch of DNA for which the starting and ending nucleotide sequences are known. It is necessary to prepare samples (plants, flower, fruit, saliva, skin) of substances whose DNA we want to replicate and we do this through a process of mushing and mixing and centrifuging. After the peparation of the samples, we prepare the gel that need to contain them. Once pipeting the samples in the gel we wait for them to give results. Already after half an hour we can understand which samples contain DNA and in which the stretch of DNA is long or short. | ||
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20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim | |||
In this workshop the engineer Lisa Thalheim introduced us the way artists made use of this new technology and showed us how to use the nanopore DNA sequencing. A small machine called MiniON that is capable of analyzing in real-time long fragments of DNA and RNA giving immediate access to results. The machine we used contains up to 512 nanopore channels for the sequencing. We prepared the sample to put in the machine and once loaded in it we compared the results with online libraries to identify the organisms present in the sample. <gallery> | In this workshop the engineer Lisa Thalheim introduced us the way artists made use of this new technology and showed us how to use the nanopore DNA sequencing. A small machine called MiniON that is capable of analyzing in real-time long fragments of DNA and RNA giving immediate access to results. The machine we used contains up to 512 nanopore channels for the sequencing. We prepared the sample to put in the machine and once loaded in it we compared the results with online libraries to identify the organisms present in the sample. <gallery> | ||
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