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</gallery> | </gallery> | ||
* Seal the jars and cover the lid with aluminum foil. | * Seal the jars and cover the lid with aluminum foil. | ||
<u>Step 3: Sterilize In Pressure Cooker</u><gallery> | <u>Step 3: Sterilize In Pressure Cooker</u> | ||
* Sterilize in the pressure cooker for 1 hour (Avoid airtight containers, as they may burst!) | |||
* Remove the lid from the cooker and let the jars cool down (Adding mycelium now could cause it to cook). | |||
* While cooling, sterilize surfaces, materials, tools, and hands with disinfectants or alcohol. | |||
<gallery> | |||
File:Preasure cooking 1.jpg | File:Preasure cooking 1.jpg | ||
File:Pressure cooking 2.jpg | File:Pressure cooking 2.jpg | ||
</gallery><u>Step 4: Add Mycelium</u><gallery> | </gallery><u>Step 4: Add Mycelium</u> | ||
* Cut a large piece of agar-mycelium from the PD and place it onto the surface of the grain (Select a piece from the outer part of the mycelium where young and fast-spreading hyphae are present). (Do not worry about transferring some agar as well; no harm will occur.) | |||
* Seal the jar with its lid. | |||
<gallery> | |||
File:Prepared mushroom 3.jpg | File:Prepared mushroom 3.jpg | ||
File:Prepared mushroom 2.jpg | File:Prepared mushroom 2.jpg | ||
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File:Cutting flowhood 1.jpg | File:Cutting flowhood 1.jpg | ||
</gallery><u>Step 5: Incubate</u> | </gallery><u>Step 5: Incubate</u> | ||
* Incubate the jar at room temperature in dark conditions. | |||
* After 3-6 days, consider gently mixing the grains to facilitate faster growth, either by using sterilized spoons to stir within the jar or by gently shaking to break up clumps. | |||
* Depending on the jar's size and the speed of growth, expect full colonization to occur within 1 to 3 weeks. | |||
For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] | For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] |
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