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* Create a ventilation hole in the lid and insert cotton wadding for mushroom air exchange. | * Create a ventilation hole in the lid and insert cotton wadding for mushroom air exchange. | ||
* Seal the jars and cover the lid with aluminum foil. | * Seal the jars and cover the lid with aluminum foil. | ||
<gallery> | <gallery widths="100" heights="100"> | ||
File:Rice solo.jpg | File:Rice solo.jpg | ||
File:Gypsum.jpg | File:Gypsum.jpg | ||
</gallery><gallery> | </gallery><gallery widths="100" heights="100"> | ||
File:Mixed rice 2.jpg | File:Mixed rice 2.jpg | ||
File:Mixed rice 3.jpg | File:Mixed rice 3.jpg | ||
File:Mixed rice.jpg | File:Mixed rice.jpg | ||
</gallery><gallery> | </gallery><gallery widths="100" heights="100"> | ||
File:Reis prep 1.jpg | File:Reis prep 1.jpg | ||
File:Reis prep 2.jpg | File:Reis prep 2.jpg | ||
File:Reis prep 4.jpg | File:Reis prep 4.jpg | ||
File:Reis prep 3.jpg | File:Reis prep 3.jpg | ||
</gallery><gallery> | </gallery><gallery widths="100" heights="100"> | ||
File:Lit-cotton.jpg | File:Lit-cotton.jpg | ||
File:Lit2.jpg | File:Lit2.jpg | ||
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* Remove the lid from the cooker and let the jars cool down (Adding mycelium now could cause it to cook). | * Remove the lid from the cooker and let the jars cool down (Adding mycelium now could cause it to cook). | ||
* While cooling, sterilize surfaces, materials, tools, and hands with disinfectants or alcohol. | * While cooling, sterilize surfaces, materials, tools, and hands with disinfectants or alcohol. | ||
<gallery> | <gallery widths="100" heights="100"> | ||
File:Preasure cooking 1.jpg | File:Preasure cooking 1.jpg | ||
File:Pressure cooking 2.jpg | File:Pressure cooking 2.jpg | ||
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* Cut a large piece of agar-mycelium from the PD and place it onto the surface of the grain (Select a piece from the outer part of the mycelium where young and fast-spreading hyphae are present). (Do not worry about transferring some agar as well; no harm will occur.) | * Cut a large piece of agar-mycelium from the PD and place it onto the surface of the grain (Select a piece from the outer part of the mycelium where young and fast-spreading hyphae are present). (Do not worry about transferring some agar as well; no harm will occur.) | ||
* Seal the jar with its lid. | * Seal the jar with its lid. | ||
<gallery> | <gallery widths="100" heights="100"> | ||
File:Prepared mushroom 3.jpg | File:Prepared mushroom 3.jpg | ||
File:Prepared mushroom 2.jpg | File:Prepared mushroom 2.jpg | ||
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|} | |} | ||
<u>Step 2: Sterilise</u> | <gallery widths="100" heights="100"> | ||
File:Mixing desk.jpg | |||
File:BaseMaterial1.jpg | |||
File:BaseMaterial3.jpg | |||
File:Mixing everything1.jpg | |||
File:Mixing everything2.jpg | |||
File:Mixing everything3.jpg | |||
File:Filling bags.jpg | |||
File:Bags1.jpg | |||
File:Filling bag6.jpg | |||
</gallery><u>Step 2: Sterilise</u> | |||
* Sterilize the bag by pressure cooking it for 50 minutes. | * Sterilize the bag by pressure cooking it for 50 minutes. | ||
* After sterilization, allow the bag to cool down. Note that the substrate can retain heat for several hours, so always squeeze it gently to assess the temperature at the center. | * After sterilization, allow the bag to cool down. Note that the substrate can retain heat for several hours, so always squeeze it gently to assess the temperature at the center. <gallery widths="100" heights="100"> | ||
File:Bags autoclayed.jpg | |||
</gallery> | |||
<u>Step 3: Mix In Mycelium</u> | <u>Step 3: Mix In Mycelium</u> | ||
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* If the liquid culture is not already fragmented, mix the contents well with a sterile or flamed spoon. | * If the liquid culture is not already fragmented, mix the contents well with a sterile or flamed spoon. | ||
* Pour or spread the inoculant into the bag and seal it. | * Pour or spread the inoculant into the bag and seal it. | ||
* Roll the bag to thoroughly mix the contents. | * Roll the bag to thoroughly mix the contents.<gallery widths="100" heights="100"> | ||
File:Bagsmixing1.jpg | |||
File:Bagsmixing2.jpg | |||
File:Bagsmixing3.jpg | |||
File:Bagsmixing5.jpg | |||
File:Bagsmixing6.jpg | |||
File:Bagsmixing7.jpg | |||
File:Bagsmixing8.jpg | |||
File:Bagsmixing9.jpg | |||
File:Bagsmixing10.jpg | |||
File:Bagsmixing11.jpg | |||
</gallery> | |||
<u>Step 4: Incubate</u> | <u>Step 4: Incubate</u> | ||