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Tardigrades documentation edited
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''' | '''To grow them in the laboratory''', we needed to reproduce their living environment and feed them. | ||
We cultivated separatel a culture of the alga "Chlorococcus" in a tube containing spring water (Volvic) and 0,01% of fertilizer (Algabloom). We kept in them in the tent for the algae, with 12h light exposure cycles. | |||
Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip): | |||
- Remove from the tardigrade culture half of the water on the top, without moving the biomass on the bottom | |||
- Replace the taken amount of water with the water coming from the Chlorococcus culture, aiming at taking the algae. | |||
- Refill the Chlorococcus culture with sterile cultivation medium (spring water with 0,01% of fertilizer algabloom) | |||
we could take care of some of them. First we disolve the initial sample which had a huge amount of tardigrades and as food they had algae. Then some of them were "sacrifice" as a contaminated sample so we could observe them through the microscope. [[File:In the lab.png|thumb|disolving the sample]]At the begining they where looking full of food but after a month in this sample we found most of the tardigrades went to sleep mode probably due to lack of food. | |||
Alessandro's input: | Alessandro's input: | ||