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GMU:Synthetic Biology/Bacteria Game/HowTo (view source)
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==Bacteria Game - How to== | ==Bacteria Game - How to== | ||
Prepare the '''agar''' with '''nutrient powder''' and fill it up with water, following the enclosed protocols. '''Boil''' the media in your microwave until the mixture turns clear and the seems to be completely dissolved. Let it cool down to approx. 50°C. Plates for the challenge have to be '''poured''' with 35 ml. The mixture can be supplied with antibiotics and inducers. Feel free to establish your own procedures. It turned out to be most precisely to inoculate by carefully pipetting 3 μl of '''liquid culture''' into the '''solidified''' agar. Nevertheless, swarm plates can also be inoculated by transferring a bacteria colony using a tooth-pick which can found in the game kit. Swarm plates are ordinarily '''incubated''' at room temperature. | Prepare the '''agar''' with '''nutrient powder''' and fill it up with water, following the enclosed protocols. '''Boil''' the media in your microwave until the mixture turns clear and the seems to be completely dissolved. Let it cool down to approx. 50°C. Plates for the challenge have to be '''poured''' with 35 ml. The mixture can be supplied with antibiotics and inducers. Feel free to establish your own procedures. It turned out to be most precisely to inoculate by carefully pipetting 3 μl of '''liquid culture''' into the '''solidified''' agar. Nevertheless, swarm plates can also be inoculated by transferring a bacteria colony using a tooth-pick which can be found in the game kit. Swarm plates are ordinarily '''incubated''' at room temperature. | ||
[[File:Figure swarm.jpg | thumb | 400px | figure: swarming bacteria (Adlung, 2010)]] | [[File:Figure swarm.jpg | thumb | 400px | figure: swarming bacteria (Adlung, 2010)]] | ||