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:'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it | :'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it | ||
:4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes | :'''4th step''': Prepare the Mannitol Soy Agar (MSA) petri dishes | ||
MSA Recipe: | ''MSA Recipe:'' | ||
In a '''250 mL conical flask''' mix: | In a '''250 mL conical flask''' mix: | ||
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*100 mL Deionised water | *100 mL Deionised water | ||
Medium alternative: Inorganic Salt Starch Agar (ISSA) | |||
:'''5th step''': Plate an aliquot of ''0.1ml of each dilution'' on the medium (MSA) | |||
:'''6th step''': Incubate at ''30° for 5-7 days'' | |||
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'''Inspiration''': | '''Inspiration''': | ||
http://annadumitriu.tumblr.com/ - Anna Dumitriu | http://annadumitriu.tumblr.com/ - Anna Dumitriu | ||
https://exploringtheinvisible.com/ - Dr. Simon Park | https://exploringtheinvisible.com/ - Dr. Simon Park |
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