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=Spot the antibiotics producer: STREPTOMYCES= | =Spot the antibiotics producer: STREPTOMYCES= | ||
[[File:Micro.png| | [[File:Micro.png|800px]] | ||
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====6th step: Incubate at 30° for 5-7 days==== | ====6th step: Incubate at 30° for 5-7 days==== | ||
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination. | '''Important:''' Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination. | ||
[[File:Incubation.png| | |||
[[File:Incubation.png|800px]] | |||
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
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==3-DAY OUTCOMES== | ==3-DAY OUTCOMES== | ||
<gallery> | |||
File:After3days.png | |||
File:After3dayss.png | |||
</gallery> | |||
'''Observations:''' | '''Observations:''' | ||
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign. | The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign. | ||
==6-DAY OUTCOMES== | ==6-DAY OUTCOMES== | ||
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File:Sample2.png|1:100 | File:Sample2.png|1:100 | ||
File:Sample3.png|1:1000 | File:Sample3.png|1:1000 | ||
File:Sample4.png|1:1000 | File:Sample4.png|1:1000 (2) | ||
</gallery> | </gallery> | ||
'''Observations:''' | |||
There has been overgrowing a hybrid of organisms, but assumingly the pinky/white powdery dots are streptomyces colonies.Such colonies are mostly to be found on the 1:100 and 1:1000 dilution plates. In this case, creating subcultures is the best solution, in order to get a unified-type of biotope in the petri dish! | |||
Only one sample has been contaminated, as it was the first one to get done during the isolation process. | |||
==FINAL DAY MICROSCOPICAL VIEWS== | |||
[[File:Micro2.png|800px]] | |||
==Insight== | |||
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing. | |||
So, next time when you get ill, decide to take another pill, in order to re-feel the thrill... | |||
'''...take a minute to think and ''praise the genus of good welfare.''''' | |||
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