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GMU:BioArt WS16/Microbiota and isolation of single bacteria (view source)
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“Kin is an assembling sort of word. All critters share a common “flesh,” laterally, semiotically, and genealogically.” (Haraway 2015) | “Kin is an assembling sort of word. All critters share a common “flesh,” laterally, semiotically, and genealogically.” (Haraway 2015) | ||
==Isolation of Gluconacetobacter xylinus== | |||
We will try isolating Gluconacetobacter xylinus, anaerobic bacteria that ferments alcohols and produces cellulose. | |||
* anahatabalance (2007) | |||
* openwetware (2015) | |||
* Kulkarni, P.K., Anil Dixit, S., Singh, U.B. (2012) | |||
===Media=== | |||
Preparation of Acetobacter Media (http://openwetware.org/wiki/Acetobacter_Xylinum_Culture) | |||
To prepare ∼500 ml of liquid Acetobacter media, add the following: | |||
*Glucose - 10 g | |||
*Peptone - 2.5 g | |||
*Yeast extract - 2.5 g | |||
*Na2HPO4 - 1.35 g | |||
*Citric acid - 0.75 g | |||
*Distilled water - 500 ml | |||
*If you are making plates, use the same protocol but add 7.5 g of agar. | |||
===Gallery=== | |||
<gallery> | |||
Image:IMG_2446.png | |||
Image:IMG_2587.png | |||
Image:IMG_0208.png | |||
Image:IMG_0210.png | |||
Image:IMG_0214.png | |||
Image:IMG_0222.png | |||
Image:IMG_0287.png | |||
Image:IMG_2440.png | |||
Image:Bacterial_pellicle_grown_in_herstin-schramm_nutrient_medium.png|Bacterial pellicle grown in herstin-schramm nutrient medium (Kulkarni et al 2012) | |||
</gallery> | |||
==References== | |||
* anahatabalance (2007). Kombucha Brewing Instructions. Available at http://www.instructables.com/id/Kombucha-Brewing-Instructions/ (accessed 29 November 2015) | |||
* [http://scialert.net/fulltext/?doi=ajdd.2012.72.86&org=10 Kulkarni, P.K., Anil Dixit, S., Singh, U.B. (2012). Evaluation of Bacterial Cellulose Produced Form Acetobacter xylinum as Pharmaceutical Excipient] | |||
* openwetware (2015). Acetobacter Xylinum Culture. Available at http://openwetware.org/wiki/Acetobacter_Xylinum_Culture (Accessed 29 November 2015) | |||