GMU:BioArt WS16/Freya Probst: Difference between revisions

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 [[File:Scobi.jpeg|500px]]
+==Serial dilution of bacteria==
+: Under the right conditions one Bacteria can divide itself infinetely and pile up a colony of its kind. The procedure of serial dilution (reducing the amount of bacteria in a solution within steps) is for example useful for counting bacteria. The Gluconacetobacter that we extracted out of the Kombucha Tea also changed its colonies with different concentration of bacteria in a solution (distilled water).
+'''Medium for the Gluconacetobacter:'''
+g Glucose
+.5 g Peptone
+.5 g Yeast extract
+.35 g Na2HPO4
+.75 g Citric acid
+ml Distilled water
+.5 g Agar
+'''1:1''' We had 6 Petridishes that contained our medium and our pipette could collect 3 ml of liquid. As a first step, we blended a piece of the Kombucha skin with 0.5 ml of distilled water and poured a drop onto our first dish.
+[[File:1-1 Gluconacetobacter xylinus.jpg|250px]]
+'''1:6''' The first 0.5 ml of bacteria-solution was diluted further more with additional 2.5 ml of distilled water. Another drop was poured onto the second dish.
+[[File:1-6 Gluconacetobacter xylinus.jpg |250px]]
+'''1:36''' We emptied the pipette again till we regained the amount of 0.5 ml and added another 2.5 ml of distilled water.
+[[File:1-36 Gluconacetobacter xylinus.jpg|250px]]
+'''1:216'''
+[[File:1-216 Gluconacetobacter xylinus.jpg|250 px]]