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→Workshop 1 (DNA samples)
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==Workshop 1 (DNA | ==Workshop 1 (DNA cloning)== | ||
*1. cutting out DNA samples from different flesh sorts (sausage) | *1. cutting out DNA samples from different flesh sorts (sausage) | ||
*2. putting samples into Eppendorf reaction tubes (Epi) | *2. putting samples into Eppendorf reaction tubes (Epi) | ||
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*11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | *11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | ||
*12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul) | *12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul) | ||
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File:pcr-IMG_4694.JPG | |||
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File:pcr-IMG_4700.JPG | |||
File:pcr-IMG_4702.JPG | |||
File:pcr-IMG_4706.JPG | |||
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File:pcr-IMG_4727.JPG | |||
File:pcr-IMG_4728.JPG | |||
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==Workshop 2 (Gel electroforese chamber)== | ==Workshop 2 (Gel electroforese chamber)== | ||