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→Workshop 1 (Molecular/DNA cloning)
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*1. cutting out DNA samples from different flesh sorts (sausage) | *1. cutting out DNA samples from different flesh sorts (sausage) | ||
*2. putting samples into Eppendorf reaction tubes (Epi) | *2. putting samples into Eppendorf reaction tubes (Epi) | ||
*3. adding buffer (1000 ul) enzyme (100 ul) in a bigger epi tube in order to prepare mix for dissolving samples (See: [[:File:DNeasy-IMG_3514.JPG]] for more info) | *3. adding buffer (1000 ul) enzyme (100 ul) in a bigger epi tube in order to prepare mix for dissolving samples (See 3-11: [[:File:DNeasy-IMG_3514.JPG]] for more info) | ||
*4. divide 200 ul of mix into tubes with samples so it dissolves all other proteins and leaves only dna | *4. divide 200 ul of mix into tubes with samples so it dissolves all other proteins and leaves only dna | ||
*5. putting samples into heating/cooling block for one hour with 56C | *5. putting samples into heating/cooling block for one hour with 56C | ||
*6. transfer liquid to the bigger epic tubes | *6. transfer liquid to the bigger epic tubes | ||
*7. adding 200 ul of | *7. adding 200 ul of solvent buffer to the dissolved samples. brakes the walls of the cell (functions as washing medium) | ||
*8. adding 200 ul 100% ethanol in order to get DNA out | *8. adding 200 ul 100% ethanol in order to get DNA out | ||
*9. transferring the liquid into the adapter tubes with powder (Ionenaustauschchromatographie) | *9. transferring the liquid into the adapter tubes with powder (Ionenaustauschchromatographie) | ||