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→Workshop 1 (Molecular/DNA cloning)
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*10. centrifuge/dremelFuge | *10. centrifuge/dremelFuge | ||
*11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | *11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | ||
*12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul) | *12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul). https://www.neb.com/protocols/2012/08/29/protocol-for-q5-high-fidelity-2x-master-mix-m0492 | ||
*PCR multiplication HOWTO: | *PCR multiplication HOWTO: | ||
a) We start at 95C two strands divides, hydrogen bonds will break at this temperature; DNA is accessible | a) We start at 95C two strands divides, hydrogen bonds will break at this temperature; DNA is accessible | ||