Bureaucrats, emailconfirmed, Administrators
5,476
edits
→Workshop 2 (Gel electroforese chamber)
| Line 116: | Line 116: | ||
*1. cutting out from plastic box | *1. cutting out from plastic box | ||
*2. preparing screws | *2. preparing screws | ||
*3. preparing 1% agarose (agarose, | *3. preparing 1% agarose (agarose, TNA, distilled water) | ||
*4. pouring agarose solution into the chamber | *4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (5ml for 100ml solution) and mixing around so it spreads. | ||
*5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | *5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | ||
*6. pouring the liquid (distiled water + the solution; no agarose) | *6. pouring the liquid (distiled water + the solution; no agarose) | ||