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→Workshop 2 (Gel electroforese chamber)
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*2. preparing screws to be put into the chamber (Fig. 2.); screws will be connected later to the power supply blue (-12V) and yellow (+12V) cables | *2. preparing screws to be put into the chamber (Fig. 2.); screws will be connected later to the power supply blue (-12V) and yellow (+12V) cables | ||
*3. preparing 1% agarose (agarose, TAE, distilled water); for 100ml solution use 1g agarose, 2ml 50x TAE buffer and 98ml distilled water | *3. preparing 1% agarose (agarose, TAE, distilled water); for 100ml solution use 1g agarose, 2ml 50x TAE buffer and 98ml distilled water | ||
*4. pouring agarose solution into the chamber and adding SERVA DNA Stain G ( | *4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (1,5ul for 100ml solution) and mixing around so it spreads. | ||
*5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | *5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | ||
*6. pouring the liquid (distiled water + the solution; no agarose) | *6. pouring the liquid (distiled water + the solution; no agarose) | ||