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Miga moved page GMU:Intro to BioArt/Freya Probst to GMU:BioArt WS16/Freya Probst
m (Miga moved page GMU:Intro to BioArt/Freya Probst to GMU:BioArt WS16/Freya Probst) |
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=Final Project= | =Final Project= | ||
== | ==Skin Variations== | ||
: | :Cellulose is generated as a byproduct of the mircroorganisms that live within Kombucha, a probiotic drink. The aim of this study was to generate skins with different properties by varying the drink or the combination of the organisms. | ||
'''1. Culture isolation of the most common microorganisms that were found in Kombucha:''' | '''1. Culture isolation of the most common microorganisms that were found in Kombucha:''' | ||
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| [[File:GAB 1-h.jpg |250px]] | | [[File:GAB 1-h.jpg |250px]] | ||
| [[File:GAB 2-h.jpg |250px]] | | [[File:GAB 2-h.jpg |250px]] | ||
| | | [[File:GAB 3-h.jpg |250px]] | ||
|- | |- | ||
| [[File:GAB 1-i.jpg |250px]] | | [[File:GAB 1-i.jpg |250px]] | ||
| [[File:GAB 2-i.jpg |250px]] | | [[File:GAB 2-i.jpg |250px]] | ||
| | | [[File:GAB 3-i.jpg |250px]] | ||
|- | |- | ||
| [[File: | | [[File:Pink dot.jpeg |250px]] | ||
| [[File:GAB | | | ||
| [[File:GAB 3-j pink dot.jpg |250px]] | |||
|- | |- | ||
|} | |} | ||
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2.7 g disodium phosphate | 2.7 g disodium phosphate | ||
1.15 g citric acid | 1.15 g citric acid | ||
{| | |||
|'''life-size''' | |||
|'''colony''' | |||
|'''oil immersion''' | |||
|- | |||
| [[File:GAB 1-k.jpg |250px]] | |||
| [[File:GAB 2-k2.jpg |250px]] | |||
| [[File:CA 3-a2.jpg |250px]] | |||
|- | |||
| [[File:GAB 1-l.jpg |250px]] | |||
| [[File:GAB 2-l.jpg |250px]] | |||
| | |||
|- | |||
| [[File:GAB 1-m.jpg |250px]] | |||
| [[File:CA 2-b.jpg |250px]] | |||
| [[File:CA 3-b red dot.jpg |250px]] | |||
|- | |||
|} | |||
| Line 251: | Line 263: | ||
2 g NaCl (salt) | 2 g NaCl (salt) | ||
30 g agar | 30 g agar | ||
{| | |||
|'''life-size''' | |||
|'''colony''' | |||
|'''oil immersion''' | |||
|- | |||
| [[File:THERMUS 1-b2.jpg |250px]] | |||
| [[File:THERMUS 2-b.jpg |250px]] | |||
| | |||
|- | |||
| [[File:THERMUS 1-c.jpg |250px]] | |||
| [[File:THERMUS 2-c.jpg |250px]] | |||
| | |||
|- | |||
| [[File:THERMUS 1-d.jpg |250px]] | |||
| | |||
| [[File:THERMUS 3-d.jpg |250px]] | |||
|- | |||
| [[File:THERMUS 1-a.jpg |250px]] | |||
| [[File:THERMUS 2-a.jpg |250px]] | |||
| [[File:THERMUS 3-a.jpg |250px]] | |||
|- | |||
| [[File:THERMUS 1-f.jpg |250px]] | |||
| [[File:THERMUS 2-f.jpg |250px]] | |||
| | |||
|- | |||
|} | |||
4. LEUCOSPORIDIELLA (fungi) -YM agar (yeast malt agar) | 4. LEUCOSPORIDIELLA (fungi) -YM agar (yeast malt agar) | ||
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20 g agar | 20 g agar | ||
{| | |||
|'''life-size''' | |||
|'''colony''' | |||
|'''oil immersion''' | |||
|- | |||
| [[File:YM 1-a.jpg |250px]] | |||
| [[File:YM 2-a.jpg |250px]] | |||
| [[File:YM 3-a.jpg |250px]] | |||
|- | |||
|} | |||
5. LACTOCOCCUS (bacteria) | 5. LACTOCOCCUS (bacteria) | ||
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(I skipped sodium acetate) | (I skipped sodium acetate) | ||
0.5 g ascorbic acid (vitamin C) | 0.5 g ascorbic acid (vitamin C) | ||
{| | |||
|'''life-size''' | |||
|'''colony''' | |||
|'''oil immersion''' | |||
|- | |||
| [[File:LACTOCOCCUS 1-a.jpg |250px]] | |||
| [[File:LACTOCOCCUS 2-a.jpg |250px]] | |||
| | |||
|- | |||
| [[File:LACTOCOCCUS 1-b.jpg |250px]] | |||
| [[File:LACTOCOCCUS 2-b.jpg|250px]] | |||
| | |||
|- | |||
| [[File:LACTOCOCCUS 1-c.jpg |250px]] | |||
| [[File:LACTOCOCCUS 2-c.jpg |250px]] | |||
| [[File:LACTOCOCCUS 3-c.jpg |250px]] | |||
|- | |||
| [[File:LACTOCOCCUS 1-d.jpg |250px]] | |||
| [[File:LACTOCOCCUS 2-d.jpg |250px]] | |||
| | |||
|- | |||
| [[File:LACTOCOCCUS 1-e.jpg |250px]] | |||
| [[File:LACTOCOCCUS 2-e.jpg |250px]] | |||
| | |||
|- | |||
|} | |||
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11 g agar | 11 g agar | ||
{| | |||
|'''life-size''' | |||
|'''colony''' | |||
|'''oil immersion''' | |||
|- | |||
| [[File:LACTOBACILLUS 1-a.jpg |250px]] | |||
| [[File:LACTOBACILLUS 2-a.jpg |250px]] | |||
| [[File:LACTOBACILLUS 3-a.jpg |250px]] | |||
|- | |||
| [[File:LACTOBACILLUS 1-b.jpg |250px]] | |||
| [[File:LACTOBACILLUS 2-b.jpg |250px]] | |||
| [[File:LACTOBACILLUS 3-b.jpg |250px]] | |||
|- | |||
| [[File:LACTOBACILLUS 1-c.jpg |250px]] | |||
| [[File:LACTOBACILLUS 2-c.jpg |250px]] | |||
| [[File:LACTOBACILLUS 3-c.jpg |250px]] | |||
|- | |||
|} | |||
'''2. Recombination of Cultures:''' | '''2. Recombination of Cultures:''' | ||
: I picked one colony from each medium and spread it on a newly cooked medium. | |||
{| | |||
|'''Sabouraud Agar''' | |||
|'''YM Agar''' | |||
|'''Thermus Medium''' | |||
|- | |||
| [[File:Sabouraud isolation.JPG |250px]] | |||
| [[File:YM Agar isolation.JPG |250px]] | |||
| [[File:Thermus isolation.jpg |250px]] | |||
|- | |||
|} | |||
{| | |||
|'''Lactococcus''' | |||
|'''Lactobacillus''' | |||
|- | |||
| [[File:Lactococcus isolation.JPG |250px]] | |||
| [[File:Lactobacillus isolation.JPG |250px]] | |||
|- | |||
|} | |||
I tried to recombine these bacteria in 16 probes, but after a week, mold spread on the liquid, because the process of fermentation was missing. | |||
[[File:Kombucha recombination.jpeg|500px]] | |||
[[File:Kombucha recombination 2.jpg|500px]] | |||
[[File:Kombucha recombination 3.jpg|500px]] | |||
'''3. Kombucha-colony in various drinks:''' | '''3. Kombucha-colony in various drinks:''' | ||
: I wondered how the choice of drink could affect the material properties of the Kombucha skin. As first reference point I looked at the ingredients of green tea and what else would contain these substances. Futhermore I did some research on where the Gluconabacter lives in nature. | |||
[[File:Drinks.jpg |500px]] | [[File:Drinks.jpg |500px]] | ||
I added 1ml Kombucha to 30 ml of an other drink, that could nourish the microorganisms. | |||
{| | |||
|'''corn juice''' | |||
|'''beer''' | |||
|'''carrot juice''' | |||
|- | |||
| [[File:Corn juice skin.jpg |250px]] | |||
| [[File:Beer skin.jpg |250px]] | |||
| [[File:Carrot skin.jpg |250px]] | |||
|- | |||
|} | |||
{| | |||
|'''superfruit juice''' | |||
|'''apple juice''' | |||
|'''coconut juice''' | |||
|- | |||
| [[File:Superfruit skin.jpg |250px]] | |||
| [[File:Apple juice skin.jpg |250px]] | |||
| [[File:Coconut juice skin.jpg |250px]] | |||
|- | |||
|} | |||