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GMU:DIY Biolab “Driver’s License”/euglena, amoeba (view source)
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A protist is any eukaryotic organism that is not an animal, plant or fungus. The protists are unicellular or unicellular-colonial and form no tissues. | A protist is any eukaryotic organism that is not an animal, plant or fungus. The protists are unicellular or unicellular-colonial and form no tissues. | ||
===Euglena=== | ===Euglena=== | ||
[[File:Euglena_wikimedia.org.jpg|400px|thumb|Euglena. Source: wikimedia.org]] | |||
====Classification==== | |||
*Domain: Eukaryota | |||
*Class: Euglenoidea | |||
*Genus: Euglena | |||
====Description==== | |||
Euglena is a (endo)symbiotic creature. It is considered belonging to protista kingdom, having features of animal, plant or fungus. | |||
“Euglena are found in fresh and salt waters. They are often abundant in quiet inland waters where they may bloom in numbers sufficient to color the surface of ponds and ditches green (E. viridis) or red (E. sanguinea).” (wikipedia) | |||
“Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia) | |||
====Medium #1==== | |||
"Combine 1 L of spring water or Chalkley’s 1x solution or Pringsheim’s, 20 wheat or rice grains, and 5 mL (1 tsp) of dry | |||
powdered milk in a beaker. Boil the mixture for 5–10 minutes, and then dilute this mixture with 3 L of spring water. Let this | |||
solution cool and stand uncovered for 24 hours. | |||
Shake or stir the media solution and fill shallow containers with wide bottoms approximately half full. (Stacking culture bowls work best.) Add 50–100 mL of Euglena stock culture to each bowl. The Euglena culture medium contains a great deal | |||
of organic matter. Bacteria will grow rapidly and the cultures are likely to have a pungent odor for several days. (Take this into | |||
consideration when selecting a culture storage location.) | |||
Chalkley’s Stock Solution 10x (Dilute by a factor of 10 for 1x (e.g., dilute 100 mL to 1 L with distilled water)) | |||
0.06 g CaCl2 | |||
1.00 g NaCl | |||
0.04 g KCl | |||
1 L Distilled water | |||
====Medium #2==== | |||
*[[:File:Euglenamedien.pdf Autotrophic and mixotrophic media]] | |||
====More info==== | |||
*[[:File:Culturing-Euglena.pdf]] | |||
===Amoeba=== | ===Amoeba=== | ||
==Microscopy== | ==Microscopy== | ||