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GMU:DIY Biolab “Driver’s License”/DNA extraction (view source)
Revision as of 15:32, 31 October 2017
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===PCR=== | ===PCR=== | ||
"Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It is an easy, cheap, and reliable way to repeatedly replicate a focused segment of DNA, a concept which is applicable to numerous fields in modern biology and related sciences." (https://en.wikipedia.org/wiki/Polymerase_chain_reaction) | "Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It is an easy, cheap, and reliable way to repeatedly replicate a focused segment of DNA, a concept which is applicable to numerous fields in modern biology and related sciences." (https://en.wikipedia.org/wiki/Polymerase_chain_reaction) | ||
Primers (molecules) forward strand and reverse strand | |||
How does it work: | |||
* We start at 95C two strands divides, hydrogen bonds will break at this temperature; DNA is accessible | |||
* Annealing: primers bind to the targets Roughly at the 65C | |||
* 72C polymerase start elongation of dna. Copies into one direction. It is called TAQ polymerase; comes from specific species called /thermus aquaticus/; lives in gazers and very stable; For every 1000 base pairs you need to copy we need around 60s. | |||
95C the started molecules bind from the other side | |||
Exponential reaction, at some point it cannot cope and more, because the volume of the epi is limited | |||
==DNA extraction== | ==DNA extraction== | ||