GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner: Difference between revisions

vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.

still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''

supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.

Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.

The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action.  But the developing organism looks rather yeasty than mycely.  

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All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination.