Bureaucrats, emailconfirmed, Administrators
5,468
edits
GMU:Board Games for Humans and Nonhumans/Photosynthetic Organisms (view source)
Revision as of 21:20, 31 January 2018
, 31 January 2018→Medium
(→Medium) |
(→Medium) |
||
| Line 72: | Line 72: | ||
====Medium==== | ====Medium==== | ||
"Combine 1 L of spring water or Chalkley’s 1x solution or Pringsheim’s, 20 wheat or rice grains, and 5 mL (1 tsp) of dry | "Combine 1 L of spring water or Chalkley’s 1x solution or Pringsheim’s, 20 wheat or rice grains, and 5 mL (1 tsp) of dry | ||
powdered milk in a beaker. Boil the mixture for 5–10 minutes, and then dilute this mixture with 3 L of spring water. Let this | powdered milk in a beaker. Boil the mixture for 5–10 minutes, and then dilute this mixture with 3 L of spring water. Let this solution cool and stand uncovered for 24 hours. | ||
solution cool and stand uncovered for 24 hours. | Shake or stir the media solution and fill shallow containers with wide bottoms approximately half full. (Stacking culture bowls work best.) Add 50–100 mL of Euglena stock culture to each bowl. The Euglena culture medium contains a great deal of organic matter. Bacteria will grow rapidly and the cultures are likely to have a pungent odor for several days. (Take this into consideration when selecting a culture storage location.) | ||
Shake or stir the media solution and fill shallow containers with wide bottoms approximately half full. (Stacking culture bowls work best.) Add 50–100 mL of Euglena stock culture to each bowl. The Euglena culture medium contains a great deal | |||
of organic matter. Bacteria will grow rapidly and the cultures are likely to have a pungent odor for several days. (Take this into | |||
consideration when selecting a culture storage location.) | |||
Chalkley’s Stock Solution 10x (Dilute by a factor of 10 for 1x (e.g., dilute 100 mL to 1 L with distilled water)) | Chalkley’s Stock Solution 10x (Dilute by a factor of 10 for 1x (e.g., dilute 100 mL to 1 L with distilled water)) | ||
| Line 83: | Line 80: | ||
0.04 g KCl | 0.04 g KCl | ||
1 L Distilled water | 1 L Distilled water | ||
Autotrophic/mixotrophic euglena medium: https://www.uni-weimar.de/kunst-und-gestaltung/wiki/File:Euglenamedien.pdf | |||
More info: [[:File:Culturing-Euglena.pdf]] | More info: [[:File:Culturing-Euglena.pdf]] | ||