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GMU:DIY Biolab “Driver’s License”/Mana Mazidy (view source)
Revision as of 01:22, 11 February 2018
, 11 February 2018→BIOLUMINESCENT BACTERIAL LIGHTBULB
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Step 1: What You'll Need | ===Step 1: What You'll Need=== | ||
Fresh (but dead) Sea fish, squid or shrimp (if you don't have access to fresh fish you can simply buy the bacteria from Carolina biological supply here: http://www.carolina.com/product/vibrio+anguillarum%2C+living%2C+tube.do?keyword=Vibrio&sortby=bestMatches) | Fresh (but dead) Sea fish, squid or shrimp (if you don't have access to fresh fish you can simply buy the bacteria from Carolina biological supply here: http://www.carolina.com/product/vibrio+anguillarum%2C+living%2C+tube.do?keyword=Vibrio&sortby=bestMatches) | ||
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Distilled Water [http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | Distilled Water [http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | ||
Step 2: Get Your Hands on Some Dead Sea Fish | ===Step 2: Get Your Hands on Some Dead Sea Fish=== | ||
<gallery> | <gallery> | ||
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===Step 3: Make Some Bacteria Food Method # 1=== | |||
Like you and me bacteria need food to grow so let's make some food that our bacteria will LOVE. Its full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live (they live in the ocean after all). | Like you and me bacteria need food to grow so let's make some food that our bacteria will LOVE. Its full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live (they live in the ocean after all). | ||
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Pour dissolved mixture of TSA, and Salt in a flask with a rag SLIGHTLY in the mouth and place in cooker. | Pour dissolved mixture of TSA, and Salt in a flask with a rag SLIGHTLY in the mouth and place in cooker. | ||
Place glass petri dishes (not needed if you bought some sterile plastic sealed ones) in as well. | |||
Fill cooker with about 1-2 inches of water. | |||
Seal pressure cooker and set heat to high | |||
When the top valve starts to dance and hiss start a timer for 15 min | |||
When time is up turn off heat and let cool down until pressure valve retracts | |||
Your agar gel and petri dishes are now completely sterilized !!! ( the steam molecules created under pressure bounces around and kills any organisms that were present) | Your agar gel and petri dishes are now completely sterilized !!! ( the steam molecules created under pressure bounces around and kills any organisms that were present) | ||
===Step 4: Bacteria Food Method # 2=== | |||
[[File:1030.jpg|400px]] [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | [[File:1030.jpg|400px]] [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | ||
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You will need: | You will need: | ||
Pancreatin (I used 325mg capsules) | *Pancreatin (I used 325mg capsules) | ||
Skim/Lowfat milk 7g | *Skim/Lowfat milk 7g | ||
100ml Distilled water | *100ml Distilled water | ||
Aquarium salt 3g | *Aquarium salt 3g | ||
Agar-Agar seaweed flakes 2g | *Agar-Agar seaweed flakes 2g | ||
Pour 7g of skim milk into a beaker. | Pour 7g of skim milk into a beaker. | ||
Add 100ml of distilled water and mix. Then add the contents of 1 pancreatin capsule and mix. | Add 100ml of distilled water and mix. Then add the contents of 1 pancreatin capsule and mix. | ||
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===Step 5: Bacteria Food Method # 3=== | |||
you can buy ready-to-pour "photobacterium" agar from Carolina Biological for about 7 bucks for 125ml....it's pricey but is the best for luminescent bacteria | |||
===Step 6: Let the Fish Glow=== | |||
[[File:1060.jpg|400px]] | [[File:1060.jpg|400px]] | ||
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I tryed to Make Some Bacteria Food : | I tryed to Make Some Bacteria Food : | ||
TSA agar medium | |||
*NaCI 2g . | |||
*Yeast Extract 1g . | |||
*Peptone 2g . | |||
*Agar 3g . | |||
*Fill with H2O up till 200 ml | |||
== Photobacterium == | == Photobacterium == | ||