GMU:DIY Biolab “Driver’s License”/Suna Yoo: Difference between revisions

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Sunayoo

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 [[:File:suna-mycelia-paper.pdf]]
+Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“.
+That day we cooked medium for euglena and amoeba, but i´d got a very bad cold, my nose was running away and i was afraid of contaminating everything just by breathing.
+[[File:CCI07012018_2.jpg|400px]]
+[[File:biolab.jpg|400px]]
+Infected by enthusiasm
+I think the first courseday i really participated. I thought about not even going to the course. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went.
+It was very interesting, Mycelium and slime molds are not as little as the other organisms. You don´t have to look through a microscope to see them. We could nearly touch them and smell them, imagine their consistence. You don´t need to have programming skills to do  art-work with them.
+Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leatherlike fabric.
+Under the instructions of Azuzena, i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. With a layer of cling film we closed the container to prevent the inside from too much contamination, but than we poked holes with a fire-desinfected needle inside. Because the mycelium needs to breathe. And then leave it in the oven, that is not called oven. Jan and i had a competition who will do it better and grow it faster. Jan won, because he cared more, i was more fascinated by Azuzena and his enthusiasm about mycelium. Jan is convinced that mycelium grows better if you love it and you show your attention to it.
+Media Mycelium grows well in:
+PDY-agar (potatoestarch-dextrose-yeast)
+coffee mold
+wood
+cardboard (it is out of wood of course..)
+straw
+Additionally it needs to be moistured regularly and grows best in a temperature between 22- 26° C. It needs also air to breathe, if you want it to crop and become fungi, it needs to be dominant over the other micro-organisms in its habit, and you have to rise the temperature i think..
+I think some humans are similar to mycelium, i could live in the same way at least for some time i think.
-.	Day in the Lab, I heard the introduction and had to leave earlier because of the Fachschaft plenum. So i didn´t learn how to cook the first medium, the one for bacteria.
+[[File:Bildschirmfoto 2018-02-11 um 13.10.37.png|400px]]
-.	Day. Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“ (1).
-That day we cooked medium for euglena and amoeba, but i´d got a very bad cold, my nose was running away and i was afraid of contaminating everything just by breathing.
-I went earlier because i was so tired, eventhough the other members of the fachschaft said that i can come later. Because uni goes first.
-[[File:CCI07012018_2.jpg|400px]]
+.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home.
-.	Day. I think the first courseday i really participated. I thought about not even going to the course, i was so stressed off my other courses, that took place the first time that week, just thinking about them made me worry about how to survive that semester. But than i quit all of them except biolab and decided to do another projekt. That was good. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went.
-It was very interesting, Mycelium and slime molds are not as little as the other organisms. You don´t have to look through a microscope to see them. We could nearly touch them and smell them, imagine their consitence. You don´t need to have programming skills to do  art-work with them.
-Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leather.
-Under the instructions of Azuzena i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. And then leave it in the oven, that is not called oven.
 [[File:PA300839.JPG|400px]]
+This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room. Azuzena told me that it could work like that.
-. Day 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room.
+So i put on my new rollerskates and desinfected the whole kitchen with Korn, a typical thuringian alcohol. Then I boiled the coffee mold for 15 minutes, maybe longer and put it in a bowl to let it cool down . Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside rotatory with pieces of the dropped spores and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air. Later that day i went to the lab to put in in the real machine. There I met Azuzena and she told me that my first experiment actually grew better than Jans.But this was only in the first place.
-So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine.
 <gallery>
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 </gallery>
+-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+Analysation of the human DNA by humans
+We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. We follow the instruction. I think we seperated our DNA...
-.	Day. We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. I don´t know what and maybe even Miga does not really know. We follow the instruction. I think we seperated our DNA..
+-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
-This is a photo of my last experiment, 1 week later:
+While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one.
-[[File:PB080889.JPG|400px]]
+ „A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia).
-I think it is contaminated, definetly too wet, says Miga.
+Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized.
-.	Day. While a PCR machine redoubles a part of our DNA, marked by a primer. Julian explains us the process (13 & 14). At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. „A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia).
-Yes and then it cools the Eppi down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough to be analized.
 Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin.
@@ Line 55: / Line 73: @@
 </gallery>
-.	Day. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why.
+-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+We wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why.
+-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+This is a photo of my last experiment, 1 week later:
+[[File:PB080889.JPG|400px]]
+I think it is contaminated, definetly too wet, says Miga.
+But how can you dry out coffee mold after cooking it (to get rid of contamination) without recontaminating it?
-.	Day. We have to present our ideas.
+We have to present our ideas.
+[[File:MycIdea1.jpg|400px]] [[File:MycIdea2.jpg|400px]] [[File:MycIdea3.jpg|400px]]
 Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process.
-Photos of that Day
-.	Day. 30.11.17  I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try.
+.11.17
+I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try.
 I don´t have Photos of that Day, but here is, what came out a week later: