GMU:Habitats SS18/Leoni Fischer

Euglena Clock

„Nothing is motionless in the eyes of the rhythmanalyst. He hears wind, rain, thunderstorm. In observing a pebble a wall or a tree he precieves the slowness of movements of these objects. Their movement is slow only in proportion to our time, our bodies, our rhythms.“

//Kurt Meyer, Rhythms, Streets, Cities

For Euglena Clock i am doing experimentation around 5 main correlations that define the concept of rhythm:

• mechanical and organic - merging technological and natural, linear and circular time
• repetition and difference - understanding Euglenas circular rhythm of time
• continous and discontinous - studying the unique rhythms of each Euglena culture
• discovery and creation - observing the rhythms of Euglena and creating a habitat for them

The goal is to create an hourglass as one of the oldest technological, linear timepieces which forms a habitat for Euglenas natural, circular rhythms of time.

• Https://upload.wikimedia.org/wikipedia/commons/thumb/0/0f/Ambrogio Lorenzetti 002-detail-Temperance.jpg/256px-Ambrogio Lorenzetti 002-detail-Temperance.jpg

Ambrogio Lorenzetti via Wikimedia commons

Euglena is a micro organism that does photosynthesis and therefore is attracted by light. In my experiments I study this phototaxis (orientation by light). For doing so I try to influence their behavior in relation to different light situations.

Experiments

Experiment1

Goal: Euglena cant recognize red light, therefore I wrapped the tube in transparent red foil to see if they would concentrate on the transparent bottom where the light source is.

Setup: Glass tube filled with water and Euglena, the tube is wrapped in semitransparent red foil. An LED light is placed on the bottom of the glass, which is not covered with foil.

Duration: 12 hours

Outcome: After 12 hours there is a visible concentration on the bottom of the tube. (Picture 3)

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Experiment 2

Goal and Setup: After seeing the concentration after 12 hours from Experiment 1 I wanted find out how long it takes to have a visible concentration of Euglena. Therefore I repeated the same experiment for 1 hour.

Outcome: After 1 hour there is already a clear distinction between the green color at the top and bottom of the glass to be made.

Duration: 1 hour

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Experiment 3

Goal and Setup: After Experiment 1 I wanted to find out to what degree gravity influenced the concentration on the bottom, therefore I made the same experiment upside down. Is the same effect occurring if only daylight is used instead of a LED?

Duration: 24 hours

Outcome: The concentration on the top of the glass is clearly recognizable. A LED light is clearly not needed. I will therefore proceed with the use of daylight.

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Experiment 4

Goal: Create a concentration thats visible through the red foil. Duration: 24hours

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Setting up sterile Euglena cultures

To avoid contamination in the final hourglass I needed to setup a new culture under sterile conditions. The recipe for doing so can be found on the frontpage of habitats.

• Picture 1: setting up fresh cultures under sterile conditions
• Picture 2: The new cultures contain roughly the same amount of euglena and are used to compare their individual time rhythms according to daylight

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  Picture 1

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  Picture 2

Setting up Euglena clock

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