GMU:Introduction to Microscopy/Denise Nicoau: Difference between revisions

GMU:Introduction to Microscopy/Denise Nicoau (view source)

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 ==='''Research: Nematodes'''===
-''In short, if all the matter in the universe except the nematodes were swept away, our world would still be dimly recognizable, and if, as disembodied spirits, we could then investigate it, we should find its mountains, hills, vales, rivers, lakes, and oceans represented by a film of nematodes.'' - by Nathan Cobb - nematologist.
+<blockquote>''In short, if all the matter in the universe except the nematodes were swept away, our world would still be dimly recognizable, and if, as disembodied spirits, we could then investigate it, we should find its mountains, hills, vales, rivers, lakes, and oceans represented by a film of nematodes.'' - by Nathan Cobb - nematologist.</blockquote>
 '''4 out of every 5 animals that live our planet are nematodes.'''
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 ''7/12/2020''
-'''Recommendations on where and how to sample:'''
+'''Recommendations on where and how to sample to find microorganisms in ''action'' by Julian Chollet:'''
+ <pre>''Always better to collect murky/turbid/not clear water and keep tubes/containers open, otherwise there might not be enough oxygen fro some microorganisms''</pre>
+* '''puddle of mud''' - mud samples, especially if taken from the bottom with some roots or plants are usually good to examine. You should add some water, shake it a bit and keep it from 2/4 days in a warm environment;
+* '''soil''' - You can find plenty of soil microbes, especially in the uppermost layer. Add some water, shake it a bit and keep it from 2/4 days in a warm environment;
+* '''pond water''' - pond samples are interesting too, where water is not clear and you can't see through, better if you take a part from the surface with a kind of film or a bigger part stored in a glass jar with also some plants/algae inside so that you can explore a kind of ecosystem.
+* '''compost''' - you can find interesting stuff also in the bottom of your garbage bin, in tree barks holes or through degrading dead plants/leaves, where you can have moisture environments and probably worms. Add some water, shake it a bit and keep it from 2/4 days in a warm environment;
+When you prepare the specimen and you have to put the slide cover do it gently, in order to prevent some microorganisms to be flattened. You can also facilitate this process by putting a hair or something else which helps to keep an empty space between cover slide and sample.