Bureaucrats, emailconfirmed, Administrators
5,468
edits
GMU:DIY Biolab “Driver’s License”/Suna Yoo (view source)
Revision as of 11:58, 28 January 2021
, 28 January 2021→Archive
No edit summary |
|||
| (26 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
===Myc Zine=== | |||
[[File:suna-myc-zine.png|600px]] | |||
<gallery> | |||
File:Bildschirmfoto 2018-03-18 um 11.53.08.png | |||
File:callforfungienglish_Seite_1.jpg | |||
File:Bildschirmfoto 2018-02-11 um 15.51.05.png | |||
</gallery> | |||
===Archive=== | |||
Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“. | Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“. | ||
That day we cooked medium for euglena and amoeba, but i´d got a very bad cold, my nose was running away and i was afraid of contaminating everything just by breathing. | That day we cooked medium for euglena and amoeba, but i´d got a very bad cold, my nose was running away and i was afraid of contaminating everything just by breathing. | ||
<gallery> | |||
File:CCI07012018_2.jpg | |||
File:biolab.jpg | |||
</gallery> | |||
===Infected by enthusiasm=== | |||
Infected by enthusiasm | |||
I think the first courseday i really participated. I thought about not even going to the course. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went. | I think the first courseday i really participated. I thought about not even going to the course. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went. | ||
| Line 17: | Line 26: | ||
Under the instructions of Azuzena, i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. With a layer of cling film we closed the container to prevent the inside from too much contamination, but than we poked holes with a fire-desinfected needle inside. Because the mycelium needs to breathe. And then leave it in the oven, that is not called oven. Jan and i had a competition who will do it better and grow it faster. Jan won, because he cared more, i was more fascinated by Azuzena and his enthusiasm about mycelium. Jan is convinced that mycelium grows better if you love it and you show your attention to it. | Under the instructions of Azuzena, i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. With a layer of cling film we closed the container to prevent the inside from too much contamination, but than we poked holes with a fire-desinfected needle inside. Because the mycelium needs to breathe. And then leave it in the oven, that is not called oven. Jan and i had a competition who will do it better and grow it faster. Jan won, because he cared more, i was more fascinated by Azuzena and his enthusiasm about mycelium. Jan is convinced that mycelium grows better if you love it and you show your attention to it. | ||
PDY-agar (potatoestarch-dextrose-yeast) | ===Media=== | ||
coffee mold | Mycelium grows well in: | ||
wood | |||
cardboard (it is out of wood of course..) | -PDY-agar (potatoestarch-dextrose-yeast) | ||
straw | |||
-coffee mold | |||
-wood | |||
-cardboard (it is out of wood of course..) | |||
-straw | |||
Additionally it needs to be moistured regularly and grows best in a temperature between 22- 26° C. It needs also air to breathe, if you want it to crop and become fungi, it needs to be dominant over the other micro-organisms in its habit, and you have to rise the temperature i think.. | Additionally it needs to be moistured regularly and grows best in a temperature between 22- 26° C. It needs also air to breathe, if you want it to crop and become fungi, it needs to be dominant over the other micro-organisms in its habit, and you have to rise the temperature i think.. | ||
I think some humans are similar to mycelium, i could live in the same way at least for some time i think. | I think some humans are similar to mycelium, i could live in the same way at least for some time i think. | ||
[[File:Bildschirmfoto 2018-02-11 um 13.10.37.png| | [[File:Bildschirmfoto 2018-02-11 um 13.10.37.png|300px]] | ||
31.10. Halloween. | |||
===Experiments=== | |||
Motivated by the experiences in the last lesson, i started doing something at home. | |||
| Line 52: | Line 69: | ||
</gallery> | </gallery> | ||
Analysation of the human DNA by humans | ===Analysation of the human DNA by humans=== | ||
We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. We follow the instruction. I think we seperated our DNA... | |||
While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | ||
„A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | |||
Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | ||
Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | ||
| Line 73: | Line 86: | ||
</gallery> | </gallery> | ||
We wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | We wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | ||
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ||
| Line 88: | Line 101: | ||
We have to present our ideas. | |||
'''We have to present our ideas.''' | |||
[[File:MycIdea1.jpg|300px]] [[File:MycIdea2.jpg|300px]] [[File:MycIdea3.jpg|300px]] | [[File:MycIdea1.jpg|300px]] [[File:MycIdea2.jpg|300px]] [[File:MycIdea3.jpg|300px]] | ||
See my presentation: [[:File:suna-mycelia-paper.pdf]] (click fast to animate it) | |||
Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | ||
| Line 100: | Line 119: | ||
I don´t have Photos of that Day, but here is, what came out a week later: | I don´t have Photos of that Day, but here is, what came out a week later: | ||
[[File:1218.JPG|400px]] | |||
This picture was my phone screen background, it took the same place as a beloved person. | This picture was my phone screen background, it took the same place as a beloved person. | ||
5 days later, it was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | 5 days later, it was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | ||
Miga and Maike said, the experiment failed, it is too dry. So i cut a stripe of it to try out the ironing theory. | Miga and Maike said, the experiment failed, it is too dry. So i cut a stripe of it to try out the ironing theory. Which i had already tried out with some dry mycelium Jan had gave me to experiment: | ||
[[File:1226.JPG|400px]] | |||
After cutting out the stripe, i folded the mycelium to the inside. Looks interesting, actually. | After cutting out the stripe, i folded the mycelium to the inside. Looks interesting, actually. | ||
And then I ironed it. | And then I ironed it. | ||
[[File:1229.JPG|400px]] [[File:1231.JPG|400px]] | |||
I didn´t want to throw away the rest of the culture, so i just cut out the contamination, to see if the mycelium survives or can take back the control. | I didn´t want to throw away the rest of the culture, so i just cut out the contamination, to see if the mycelium survives or can take back the control. | ||
| Line 119: | Line 142: | ||
</gallery> | </gallery> | ||
Therefore i cooked some old potatoes | |||
I went to the Lab to make my own PDA-medium. To experiment more with mycelium and find out wich are the best conditions to grow it. Because i think for the paper production i need a lot more mycelium, i needs to be well moisture, for a stable growth, but nor to much medium, so that it can eat up the whole of it and i don´t have to dissolve the medium from the mycelium, because the water is not good for the mycelium either. | |||
Therefore i cooked some old potatoes, and boiled them for maybe 20 minutes, than took them out of the water. Weighed the Agar and the Dextrose, and added them to the Starch- brew. It burned a little on the ground of the pot, because the sugar can´t get too hot. | |||
<gallery> | <gallery> | ||
File:Potatoes-cooking.JPG | File:Potatoes-cooking.JPG | ||
File:Taking-them-out.JPG | |||
File:Agar&Dextrose.JPG | |||
File:Burned-Pot.JPG | |||
</gallery> | </gallery> | ||
Meanwhile i had cleaned the "Telstar" disinfected it and a lot of petridishes with alcohol and UV-light. Prepared everything to be ready for the medium. | Meanwhile i had cleaned the "Telstar" disinfected it and a lot of petridishes with alcohol and UV-light. Prepared everything to be ready for the medium. | ||
I filled them and let them cool down with the fan for some minutes. | |||
I waited. | |||
[[File:Filled-Petridishes.JPG|400px]] | [[File:Filled-Petridishes.JPG|400px]] | ||
<gallery> | <gallery> | ||
| Line 173: | Line 188: | ||
</gallery> | </gallery> | ||
This is what you could see 5 Days later: | |||
<gallery> | <gallery> | ||
| Line 200: | Line 215: | ||
I thought, now its proven: the closet is better than the oven! But Maike said, maybe the process in the oven is just fastened and the contamination sleeps in the closet-petridishes as well. So i decided to wait one more week. To see what happens. | I thought, now its proven: the closet is better than the oven! But Maike said, maybe the process in the oven is just fastened and the contamination sleeps in the closet-petridishes as well. So i decided to wait one more week. To see what happens. | ||
One week later was the 28.12.17. I was in YMR for exactly 1 Day, and although i borrowed the key from Tseh, i couldn´t enter the BioLab because the whole building was closed and the Thoska didn´t work. | |||
10.01.2018 | |||
I just took pictures of the petridishes: | |||
<gallery> | <gallery> | ||