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Based on the above experiment, I came up with an idea to observe these little creatures. | Based on the above experiment, I came up with an idea to observe these little creatures. | ||
When I was raising Physarum | When I was raising Physarum Polycephalum, I found that they didn't like coffee. What if I add coffee to their growth medium, will they adapt to such an environment? | ||
I decided to split the slime into two parts. One is fed with a normal 2% agar medium. In the other one's medium, I will replace the water with an appropriate amount of coffee. In the beginning, the amount of coffee is small, allowing slimes to grow on it. After that, the amount of coffee will be increased after every time the slime adapts to the environment of the medium. Then let the slime that can grow on the coffee medium and the slime that have not lived on the coffee medium live together on the ordinary agar medium for a period of time. Finally, the slimes who have lived together for a period of time are raised in a coffee medium to observe whether they can accept the coffee environment. | I decided to split the slime into two parts. One is fed with a normal 2% agar medium. In the other one's medium, I will replace the water with an appropriate amount of coffee. In the beginning, the amount of coffee is small, allowing slimes to grow on it. After that, the amount of coffee will be increased after every time the slime adapts to the environment of the medium. Then let the slime that can grow on the coffee medium and the slime that have not lived on the coffee medium live together on the ordinary agar medium for a period of time. Finally, the slimes who have lived together for a period of time are raised in a coffee medium to observe whether they can accept the coffee environment. | ||