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== Notebook == | == Notebook == | ||
* usage of [http://2009.igem.org/Team:Cambridge E. chromi principle] (pigmented ''E. coli'' cells) | |||
* testing of a synthetic ''E.coli'' predator-prey system ([http://www.nature.com/nchembio/journal/v5/n12/pdf/nchembio.244.pdf Song ''et al.'', 2009]), ([http://www.nature.com/msb/journal/v4/n1/pdf/msb200824.pdf Ballagaddé ''et al.'', 2008]) | |||
=== 29/10/2010 === | === 29/10/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective LB agar plates (10 g Tryptone, 5 g Yeast extract, 10 g NaCl and 15 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics) | * over-night growth on selective LB agar plates (10 g Tryptone, 5 g Yeast extract, 10 g NaCl and 15 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics) | ||
{| class="wikitable sortable" , border="1" | {| class="wikitable sortable" , border="1" | ||
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* glycerol stocks (prepared directly after arrived) from predator and prey cells (see table 2) were plated on selective LB agar plates (s. a.) | * glycerol stocks (prepared directly after arrived) from predator and prey cells (see table 2) were plated on selective LB agar plates (s. a.) | ||
{| class="wikitable sortable" , border="1" | {| class="wikitable sortable" , border="1" | ||
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| style="font-weight:bold;" | 3a || prey || MG1655 || pLasRLuxI-luxCcdBs, ptet-mCherry(ColE1) || Cm, Kan | | style="font-weight:bold;" | 3a || prey || MG1655 || pLasRLuxI-luxCcdBs, ptet-mCherry(ColE1) || Cm, Kan | ||
|} | |} | ||
=== 30/09/2010 === | |||
* inoculation of day cultures with colonies from the previous day | |||
** in 5 mL LB + required antibiotic | |||
* preparation of TB soft agar plates (10 g Tryptone, 5 g NaCl and 3 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics swarm plates) for swarming | |||
== |
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