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After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it. | After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it. | ||
== | == A visual representation of bacterial communication systems == | ||
# | # Streptomyces isolation from pure soil | ||
I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | |||
II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Agar (MSA)''' | |||
III. Incubate at '''30°C for 7 days''' | |||
: | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
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# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient. |
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