GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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===ISOLATION OF STREPTOMYCES=== | ===ISOLATION OF STREPTOMYCES=== | ||
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | ====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | ||
:[[File:Soil.png|200x]] | |||
====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min==== | ====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min==== |
Revision as of 22:51, 12 July 2016
Spot the antibiotics producer: STREPTOMYCES
Antibiotics.
The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.
It’s simply the recuperation cycle.
But, do you ever think about the fundamentals of having the chance of recuperation from infections?
Sure.
It’s thanks to the pill - the antibiotic.
Good job!
Now, zoom in deeper…
Do you actually know where this savior comes from?
Think no further than the soil.
Streptomyces.
ISOLATION OF STREPTOMYCES
1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment
2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min
3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it
4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes
MSA RECIPE: In a 250 mL conical flask mix: 2g Agar 2g Mannitol 2g Soya flour (Holland and Barrett) 10 mM CaCl2 100 mL Deionised water :[[File:Mannitol.png]] Medium alternative: Inorganic Salt Starch Agar (ISSA)
5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)
6th step: Incubate at 30° for 5-7 days
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park