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====6th step: Incubate at 30° for 5-7 days==== | ====6th step: Incubate at 30° for 5-7 days==== | ||
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination. | |||
[[File:Incubation.png|400px]] | [[File:Incubation.png|400px]] | ||
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
---- | ---- | ||
==3-DAY OUTCOMES== | ==3-DAY OUTCOMES== | ||
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]] | ::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]] |
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