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→Workshop 1 (Molecular/DNA cloning)
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*8. adding 200 ul 100% ethanol in order to get DNA out | *8. adding 200 ul 100% ethanol in order to get DNA out | ||
*9. transferring the liquid into the adapter tubes with powder (Ionenaustauschchromatographie) | *9. transferring the liquid into the adapter tubes with powder (Ionenaustauschchromatographie) | ||
*10. | *10. centrifuge/dremelFuge | ||
*11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | *11. 3 centrifuge cycles with different buffer; the last one with water in order to have dna in the final sample | ||
*12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul) | *12. polymerase master mixer (half of the total mix; 15ul; from one dna makes more dna) + sample (10ul) + primer forward (2,5ul) + primer reverse (2,5ul) | ||