GMU:BioArt WS16/Fernanda Caicedo

KOMBUCHA TEA

Results of making KOMBUCHA TEA

Drying up Scoby to preserve

ISOLATING BACTERIA from Scoby

Preparation of Acetobacter Media

Mixing Media with Agar

Boiling Media

Putting up Media into dishes

Result after one week

GROW BACTERIA FROM BODY

Result afther 2 weeks

Wiew using the Microscope

Making Macrophtography

MICROSCOPE: Oil immersion

Procedure:

GROWING FUNGI

Procedure:

MAKING BACTERIA BATTERY

Procedure:

== BioArt Projeckt ==

MICRO RITUALS

The Earth has evolved through the exercise of error and consciousness. Man himself is only flesh, matter that becomes living energy by assimilating his mystical and cosmic form. Human behaviour has the power to transform the biosphere. As a species man is the greatest existing biological force: it consumes, alters, transforms, creates and destroys. It increasingly resembles to an insatiable bacteria of resources, territory and power, strengthening its destructive nature and minimizing its creative nature by its predatory instinct. This project is a personal discovery in which my cultural roots and current state of consciousness are the protagonists. Through the magnification of the imperceptible, I intend to open a discussion in an archetypal and symbolic way about the essence and purpose of human life, with the help of images, building a bridge of symbiotic relationships between past, present and future.

Past: Represented by geometric schemes of animals originated from the indigenous culture of “Salasaca” of the central zone of Ecuador (Province of Tungurahua). In the Andean worldview, art is one of the essential forms of transmission of culture and ancestral teachings, is also an identification tool and a vehicle of expression. Indigenous culture is characterized by its conscientious relationship of dependence with the forces of nature. Getting retroactive-feedback through conscious energy achieved with a lifestyle close to nature and grace rituals that include offerings.

Present: Myself and my being, represented by my body. My present external condition, the skin I inhabit; in which allows me to interact in this physical reality with other life forms that adapt in symbiosis to survive.

Future: Bacteria that inhabit my skin as anonymous guests on scales almost impossible for my understanding. According to science these are beings provided with special conditions in the universe, because they are able to adapt, unite, mutate and survive in any existing environment.

Ritual The consciousness about my being and its place in this frequency connects me directly with life. It awakens my sensory sensibility in such a way that it makes me part of the cosmic energy in the great universal mystic temple, which I thank with these actions, as my ancestral culture would have done: using art as a ritualistic means of expression and life. Action in which I give life to these ancestral schemes that are symbols enclosed and transmitted through the feeling of time, space, and reality.

First attempt STENCIL

== Process: ==

1 Step:

MEDIA

-Gluose -Peptone -Yeast extract -Distilled Water -Agar

Growing my own Bacteria

Bacteria Eyes

Face Bacteria

2 Step:

Cut the pattern on a metallic surface.

"Salasaca Bird" Stencil Pattern

Pattern trimmed in a solution (100ml) of 1,5 g Agar + distilled water 100ml

Trimmed Agar fulled with solution (100ml) of peptone 0,5 g + agar 1,5 g + sugar 2 g and yeast 0,5g

Results after 7 days of placing the bacteria in the stencil area

Second attempt DRAWING WITH BACTERIA

Process:

Using the bacteria-free area

Using my eyes bacteria for drawing

Result after 7 days

== Third Attempt DRAWING ON STENCIL AREA ==

Process:

Using my Face bacteria after 14 days of incubation

Using an Agar base

Adding the stencil on the Agar platte

Waiting till it draws

Cutting the stencil area

Fulling trimmed Agar with mix of peptone, agar, sugar and yeast

Drawing on the stencil area with my face bacteria

RESULTS AFTHER 5 DAYS

RESULTS AFTHER 15 DAYS

'Fourth attempt

Materials

MEDIA 1 100ml

-Antibiotic 0,30 g -Distilled water 100ml -Agar 1,5 g

MEDIA 2 100ml

-Glucose 2g -Peptone 0,5 g -Yeast extract -Distilled water 100ml -Agar 1,5 g

MEDIA 1 100ml

MEDIA 2 100ml

Results after 8 Days

In this case no bacteria grow but fungi from air.

CONCLUSIONS:

Use less Antibiotic and draw the bacteria with some tweezers using already growing bacteria. Be careful with the disinfected working area.

== Fifth attempt ==

After several times of experimenting with deferent types of nutrients and ways of applying the bacteria. I finally reach the expected result:

Experiment:'

Materials

- 3 clean and disinfected petra dishes

- 2 nutrient boiling containers

- 1 pipet

- 2 blades

- 3 cotton swabs

- 1 tweezers

- 20 cubic centimeters foil

The experiment will need 2 types of Agar.

AGAR 1(100ml Nutrient): 100ml distilled water - 0,32g disinfectant - 2g Agar.

AGAR 2 (100ml Nutrient): 100ml distilled water - 2g glucose - 0,5g peptone - 0,5g yeast extract - 0,27g Na2HPO4 - 2g Agar.

ALL THE EXPERIMENT SHUND BE DEVELOP IN THE INFECTION FREE AREA!

Step 1

Cut the schemes on the foil and preserve in alcohol.

Step 2

Prepare AGAR 1, mix and heat until boiling. Put the AGAR 1 into the 3 disinfected petra dishes, add the schemes on the top, cover and wait until it draws.

Step 3

with the help of the blades and the tweezers cut the edges of the schemes in order to empty the design, cover and preserve.

Step 4

Prepare AGAR 2, mix and heat until boiling. With the help of the pipet place the AGAR 2 into the empty places of the petra dishes, cover and preserve until it draws.

Step 5

Take bacteria from your skin with the cotton swabs and draw carefully into the AGAR 2 area, cover it and let it rest for about 8 days.

SUCCESS¡

CLONING EXPERIMENT