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GMU:BioArt WS15/Transgenic Organism. Plant cloning (view source)
Revision as of 15:53, 10 October 2017
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=== mythological hybrids === | === mythological hybrids === | ||
Pegasus, Centaur, mermaids, Minotaur. “In mythology, folklore and speculative fiction, shapeshifting, or metamorphosis is the ability of an entity to physically transform into another being or form. This is usually achieved through an inherent faculty of a mythological creature, divine intervention, or the use of magic spells or talismans.”(wikipedia) | Pegasus, Centaur, mermaids, Minotaur. “In mythology, folklore and speculative fiction, shapeshifting, or metamorphosis is the ability of an entity to physically transform into another being or form. This is usually achieved through an inherent faculty of a mythological creature, divine intervention, or the use of magic spells or talismans.”(wikipedia) | ||
<gallery> | |||
Image:Chimera_d'arezzo,_fi,_04.png|Source: Wikipedia | |||
Image:Minotauros_Myron_NAMA_1664_n1.png|Source: Wikipedia | |||
</gallery> | |||
===Selective breeding=== | ===Selective breeding=== | ||
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"The foreign DNA may be expressed as extrachromosomal satellite DNA or it may be integrated into the cellular chromosomes. Every living organism has a genetic code that can be manipulated, and the recombinant DNA can be passed on to the next generations." (Kac 1998) | "The foreign DNA may be expressed as extrachromosomal satellite DNA or it may be integrated into the cellular chromosomes. Every living organism has a genetic code that can be manipulated, and the recombinant DNA can be passed on to the next generations." (Kac 1998) | ||
"Among the most important tools in the genetic engineer's tool kit are enzymes that perform specific functions on DNA. .. enzymes known as ligases join the ends of two DNA fragments. These and other enzymes enable the manipulation and amplification of DNA, essential components in | |||
joining the DNA of two unrelated organisms."(Fenwick 2004) | |||
=== Methods for genetically modified plants === | === Methods for genetically modified plants === | ||
* man mischt vereinzelte Zellen mit DNA Molekülen | * man mischt vereinzelte Zellen mit DNA Molekülen | ||
* Agrobacterium tumefaciens (Boden Bakterien, die Bakterien kommen aus der Natur, sie greifen die Pflanzen an wenn die verletzt sind; die infizieren die Pflanzen mit DNA; damit entsteht ein Tumor/ | * Agrobacterium tumefaciens (Boden Bakterien, die Bakterien kommen aus der Natur, sie greifen die Pflanzen an wenn die verletzt sind; die infizieren die Pflanzen mit DNA; damit entsteht ein Tumor/callus; durch diese Bakterien genetisch verändert werden; die Bakterien haben in der Zeit ein Gen/Bauplan für ein eiweiss, damit die pflanzen dieses Eiweiß produzieren und die Bakterien fressen da; Kwass Nahrung mittel; man nennt dieses Phänomen genetische Kolonisation). Zufall. Bakterien machen Verbindungskanäle mit Injektion von DNA. Woher kann man die DNA Teile bestimmen | ||
* CRISPR/Cas9. Methode damit man ganz genau bestimmen wo die neue DNA eingebaut wird | * CRISPR/Cas9. Methode damit man ganz genau bestimmen wo die neue DNA eingebaut wird | ||
== | == Plant cloning HOWTO== | ||
*pflanzen Blatter ins enyzm geben ( | man kann von einer Pflanze Millionen klonierten Pflanzen züchten. das wichtigste ist eine Zelle zu haben. | ||
[[File:Transgenic_Crops.pdf]] | |||
*pflanzen Blatter ins enyzm geben (Cellulase Macerozyme) | |||
*enzyme kommen von microorganismen und die teilen die zellen (fressen zellenwände) | *enzyme kommen von microorganismen und die teilen die zellen (fressen zellenwände) | ||
*zellverband zerstören und auflösen damit wir einzelne Zellen naher haben | *zellverband zerstören und auflösen damit wir einzelne Zellen naher haben | ||
*wenn das Milieu lebend/gut/günstig ist, können wir diese Zellen durch Teilung vermehren | *wenn das Milieu lebend/gut/günstig ist, können wir diese Zellen durch Teilung vermehren | ||
*am Anfang sieht man | *am Anfang sieht man Callus (undifferenziertes pflanzen Gewebe) | ||
*man braucht bestimmte pflanzenhormonmischung um daraus Mehrstoff | *man braucht bestimmte pflanzenhormonmischung um daraus Mehrstoff | ||
*um ein Richtiges/siehtbares Pflanzen zu haben braucht man schon ein paar Monate. Und dafür sollte bestimmtes PH, Temperatur, Licht usw stehen | *um ein Richtiges/siehtbares Pflanzen zu haben braucht man schon ein paar Monate. Und dafür sollte bestimmtes PH, Temperatur, Licht usw stehen | ||
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*iGEM Competition. The iGEM Competition is the premiere student competition in Synthetic Biology. Since 2004, participants of the competition have experienced education, teamwork, sharing, and more in a unique competition setting. Multidisciplinary teams work all summer long to build genetically engineered systems using standard biological parts called Biobricks. http://igem.org/ | *iGEM Competition. The iGEM Competition is the premiere student competition in Synthetic Biology. Since 2004, participants of the competition have experienced education, teamwork, sharing, and more in a unique competition setting. Multidisciplinary teams work all summer long to build genetically engineered systems using standard biological parts called Biobricks. http://igem.org/ | ||
*Super Cell. The project Super Cell is a fictional supermarket offering speculative products, which are all based on Synthetic Biology. The projects aims to improve public awareness about Synthetic Biology and at the same time, foster a debate on how we want to see it manifested in our everyday lives. Implications of Biotechnologies are usually discussed on a philosophical, ethical, legal or possibly religious level. https://vimeo.com/39653216 http://super-cell.org/ | *Super Cell. The project Super Cell is a fictional supermarket offering speculative products, which are all based on Synthetic Biology. The projects aims to improve public awareness about Synthetic Biology and at the same time, foster a debate on how we want to see it manifested in our everyday lives. Implications of Biotechnologies are usually discussed on a philosophical, ethical, legal or possibly religious level. https://vimeo.com/39653216 http://super-cell.org/ | ||
<gallery> | |||
Image:albagreen.png|GFP Bunny. Source: ekac.org | |||
Image:kac.nat.hist.enigma.01.png|Edunia. Source: ekac.org | |||
</gallery> | |||
== References == | == References == | ||