|
|
| Line 82: |
Line 82: |
| ===DNA-Extraktions-Kit Protocol=== | | ===DNA-Extraktions-Kit Protocol=== |
| [[File:miga-IMG_3514.JPG|400px]] | | [[File:miga-IMG_3514.JPG|400px]] |
|
| |
| ==PCR==
| |
| Primers (molecules) forward strand and reverse strand
| |
|
| |
| How does it work:
| |
| * We start at 95C two strands divides, hydrogen bonds will break at this temperature; DNA is accessible
| |
| * Annealing: primers bind to the targets Roughly at the 65C
| |
| * 72C polymerase start elongation of dna. Copies into one direction. It is called TAQ polymerase; comes from specific species called /thermus aquaticus/; lives in gazers and very stable; For every 1000 base pairs you need to copy we need around 60s.
| |
|
| |
| 95C the started molecules bind from the other side
| |
|
| |
| Exponential reaction, at some point it cannot cope and more, because the volume of the epi is limited
| |
