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m (Miga moved page GMU:Intro to BioArt/Transgenic organisms to GMU:BioArt WS16/Transgenic organisms) |
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===GloFish=== | ===GloFish=== | ||
https://www.google.de/search?q=GloFish | https://www.google.de/search?q=GloFish&tbm=isch | ||
The first genetically modified animal to be sold as a pet | The first genetically modified animal to be sold as a pet | ||
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Needed: | Needed: | ||
* ( | * (Reptile) incubator | ||
* Spectrometer to calculate cell growth mass | * Spectrometer to calculate cell growth mass | ||
TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water. | TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water. |