GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project: Difference between revisions
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'''Abstract''' | '''Abstract''' | ||
Specimen of mushrooms obtained from weimerian supermarkets are cloned and purified, than stored in a cold-storage strain-bank. | Specimen of mushrooms obtained from weimerian supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. | ||
Via reverse-engineering | Via reverse-engineering the strand from a commercial product is extracted and cultured. The strands will be available at an exhibition. | ||
'''Method''' | '''Method''' | ||
For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag. | |||
The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, | |||
The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till | '''References''' | ||
Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) | |||
1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 | |||
Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7 | |||
Revision as of 19:33, 28 January 2018
Abstract
Specimen of mushrooms obtained from weimerian supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strand from a commercial product is extracted and cultured. The strands will be available at an exhibition.
Method
For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag.
References
Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7