GMU:DIY Bio: doing things with biology/Antje Danz: Difference between revisions

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=='''1st class: 16. Oktober 2018'''==
=='''1st class: 16. Oktober 2018 - Introduction'''==


===Summary===
===Summary===
Preparing the first testing '''Agar-Plates''' for bacterial growth in a petri dish:
General introduction to working in the bio lab. Preparing the first testing '''Agar-Plates''' for bacterial growth in a petri dish:
*petri dishes where filled with 15 ml of an [[GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools|LBP Medium]] with Agar Agar
*petri dishes where filled with 15 ml of an [[GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools|LBP Medium]] with Agar Agar
*cooling down
*cooling down

Revision as of 12:49, 22 October 2018

1st class: 16. Oktober 2018 - Introduction

Summary

General introduction to working in the bio lab. Preparing the first testing Agar-Plates for bacterial growth in a petri dish:

  • petri dishes where filled with 15 ml of an LBP Medium with Agar Agar
  • cooling down
  • inoculateing with different surface smear
    • In my case: human nose (inside), cat nose (outside) and fur, fridge glass surface and 1 cent coin
  • left with room temperature for the following days

First culture medium

As described obove the filled petri dishes are no beeing observed for the following days.

Gallery

Breeding day 7 - closer look

Somehow I got the impression that the petri dishes weren't sterile as after 7 days almost the full dish is covered with a white film. You can only guess by slightly different colors where I initially inoculated the dish. Something seems to grow over the originally inoculated sections. See below some close ups. In the section of the example I took from my nose, a small yellow dot appears. I think this is what was intended to be growing. The rest just looks almost the same.

Gallery

DIY Microscope Test

What was done?

  • extracted converging lens from a laser pointer
  • attached to iPhone SE back camera

Results

  • adding some filters to increase contrast und depth

2nd class: 23. October 2018

Summary